丙酮酸乙酯对内毒素性急性肺损伤大鼠肺组织巨噬细胞炎症蛋白-2表达的影响  

Effects of ethyl pyruvate on the expression of chemokine macrophage inflammatory protein 2 in thelung induced by endotoxin in rat

作　　者：季艳梅[1] 郑翔[1] 郭家龙[2] 刘伯毅[1] 

机构地区：[1]郧阳医学院附属太和医院中心ICU科,湖北十堰442000 [2]华中科技大学同济医学院附属协和医院胸心外科,430022

出　　处：《中国急救医学》2009年第1期53-55,共3页Chinese Journal of Critical Care Medicine

基　　金：湖北省科技攻关项目（No.2004AA301C97）

摘　　要：目的观察丙酮酸乙酯（EP）预先给药对内毒素性急性肺损伤（ALI）大鼠肺组织巨噬细胞炎症蛋白-2表达的影响，探讨丙酮酸乙酯可能的保护机制。方法静脉注射脂多糖（LPS）5mg／kg，复制大鼠ALI模型。雄性SD大鼠30只随机分为三组：A组为对照组，B组为LPS组，C组为EP＋LPS组，于静脉注射LPS前1h腹腔内注射EP（40mg／kg）。所有动物于注射LPS或生理盐水后6h颈动脉放血处死，RT-PCR法测定肺组织巨噬细胞炎症蛋白-2mRNA的表达；酶联免疫吸附法测定肺组织TNF-α和IL-1β的含量。结果与A组相比，B组、C组肺组织巨噬细胞炎症蛋白-2mRNA表达增加，肺组织TNF-α和IL-1β含量上升（P〈0．05）；与B组相比，C组肺组织巨噬细胞炎症蛋白-2mRNA表达降低，肺组织TNF-α和IL-1β含量降低（P〈0．05）。结论丙酮酸乙酯通过下调大鼠LPS诱导的肺组织巨噬细胞炎症蛋白-2表达，降低了TNF-α和IL-1β的释放，减轻ALI大鼠肺部的炎症反应。Objective To investigate the effects of ethyl pyruvate （EP） pretreatment on the expression of macrophage inflammatory protein 2 （ MIP - 2 ） and content of tumour necrosis factor - alpha { TNF - α） and interleukin - 1 （ IL - 1β ） in the lungs induced by lipopolysaccharide I LPS }, and to explore the mechanisms of ethyl pyruvate protectjng the lungs. Methods The rat model of acute lung injure was established via femoral vein injection lipopolysaccharide（ LPS,5 mg/kg}. Thirty male SD rat weighing 200 - 250 g were randomly divided into 3 groups （n = 10 each ） ：group A control;group B LPS;group C EP ＋ LPS, Group A received physiologic saline 30 mg/kg only, while group B received 0.1% LPS 5 mg/ kg and physiologic saline 30 mg/kg by right femoral vein injection. The treatment of group C is same with group B except that it pretreated with intrapentoneal EP（40 mg/kg） 4ml before 1 h of LPS injection. The animals were bled to death at 6 h after LPS administration. The lungs were immediately removed for determination of expression of MIP - 2mRNA （ reverse transcription - polymerasechainre - action, RT - PCR ） and TNF - α and IL - 1β content （ enzymelinked immunosorbentassy, ELISA ） in the lung. Results Compared with group A, the expression of MIP - 2mRNA in the cytoplasm and the level of TNF - α and IL - 1β in the lungs was increased in group B and group C ; while compared with group B, they were decreased in group C （ P 〈 0.05 }. Conclusion EP can attenuate the changes in MIP - 2 expression and TNF - α and IL - 1β content in the lung induced by LPS and may alleviate the inflammation of acute lung injury in rat.

关 键 词：丙酮酸盐类 脂多糖类 巨噬细胞炎症蛋白-2 

分 类 号：R563[医药卫生—呼吸系统]