维甲酸对人成骨肉瘤MG-63细胞增殖和相关基因表达的影响  

Effects of Retinoic Acid on Proliferation and Associated Gene Expression of Human Osteosarcoma MG-63 Cells

作　　者：宋建晔[1] 赵妍[1] 李祺福[1] 石松林[1] 赵振利[1] 荆光军[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学研究室,福建厦门361005

出　　处：《现代生物医学进展》2009年第1期5-8,F0002,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30470877)

摘　　要：目的:研究维甲酸对人成骨肉瘤MG-63细胞增殖和相关基因表达的影响,以探索其对成骨肉瘤细胞的生物学效应。方法:以1μmol/L维甲酸处理人成骨肉瘤MG-63细胞,生长曲线测定,流式细胞仪分析、光镜观察和免疫细胞化学检测等研究维甲酸对MG-63细胞的生长曲线、细胞周期和相关癌基因、抑癌基因表达的影响,并对其作用机理进行初步分析。结果:维甲酸处理7天后,MG-63细胞生长抑制率达到42.2%,G0/G1期比例达到61.8%,细胞形态铺展,排列趋于规则,癌基因c-myc、c-fos的表达降低,而抑癌基因Rb、p27表达上调。结论:1μmol/L维甲酸可以有效抑制细胞的增殖活动,改变细胞恶性形态特征,下调癌基因c-myc、c-fos和上调抑癌基因Rb、p27的表达,从而对人成骨肉瘤细胞分化具有诱导作用。Objective： To explore effect of Retinoic Acid （RA） on proliferation of human osteosarcoma MG-63 cells and its mech- anism of antitumor. Methods： The MG-63 cells treated by 1 μmol/L RA were subjected to growth curve counts, flow sytometry, immuncocytochemical assay and light microscopy. We investigated the influence of RA on proliferation, cell cycle, expression of associated oncogene and tumor suppressor gene of MG-63 cells. Then we analyzed the mechanism of RA in inducing differentiation primarily. Results： Seven days after treated by 1 μmol/L RA, the inhibition rate of proliferation of MG-63 cells amounted to 42.2 % and RA reduced the rate of progression from G1 to S phase. The cells tend to be flat and spread. Meanwhile, the expression level of oncogene was down- regulated and the expression level of tumor suppressor gene was upregulated. Conclusions： 1μmol/L RA could inhibit proliferation of MG-63 cells, reverse the malignant phenotype characters, downregulate expression of Oncogene c-Myc, c-fos and upregulate expression of Tumor Suppressor Gene Rb, p27, as a result, induce MG-63 cells into differentiation effectively.

关 键 词：MG-63细胞 维甲酸 细胞增殖 癌基因 抑癌基因 

分 类 号：Q254[生物学—细胞生物学] Q279