C-MET在大鼠胰腺发育过程中的表达  

Expression of C-MET During Development of Rat Pancreas

作　　者：龚华[1] 张国英[1] 蔺扬波[1] 程梅[1] 邵秀琴[1] 戴岭[1] 高丽[1] 袁栎[1] 张一鸣[1] 德伟[1] 

机构地区：[1]南京医科大学分子生物化学系,江苏南京210029

出　　处：《现代生物医学进展》2009年第1期16-19,共4页Progress in Modern Biomedicine

基　　金：国际科技合作计划(BZ2005043)

摘　　要：目的:研究C-MET在大鼠胰腺发育阶段的表达和细胞定位。方法:运用RT-PCR和Western Blot技术分别检测C-MET在大鼠胰腺发育阶段的mRNA和蛋白表达水平;运用免疫组化和免疫荧光技术检测不同时期C-MET在胰腺的组织细胞学定位。结果:RT-PCR结果显示E18.5C-MET mRNA高表达。Western Blot结果显示其蛋白在P14,P21高表达,并存在两种亚型,分子量分别为190KD和170KD。免疫组化和免疫荧光结果显示在不同发育时期C-MET在胰岛β细胞和间充质细胞都有表达。结论:C-MET在大鼠胚胎发育后期及生后出现高表达,并表达于胰岛β细胞和间充质细胞,可能参与了胰岛形成、结构重塑和功能维持。Objective： To research the expression and the cell location of C-MET during pancreas development. Methods： RT-PCR, Western Blot, immtmo-histochemical stain and double fluorescence immunohistochemistry were used to check C-MET expressions on mRNA and protein levels and the location in pancreas respectively in different stage of pancreas development. Results： The expression of C-MET was high significantly in E18.5, reaching the peak in P21, and then decreased until adult rat pancreas. C-MET protein expression tendency was consistent with the C-MET mRNA expression. C-MET presents two specific subtypes in the developmental pancreas（molecular weight： 190 KD and 170KD）. Immunolocalization indicated that C-MET not only colocalized with insulin in islet beta-cells but also colocalized with vimentin in mesenchyme of developing rat pancreas. Conclusion： C-MET is expressed highly in islet beta-cells and mesenchymal cells in rat Pancreas in the later stage of development and after birth, which may be related to formation and remodeling of pancreatic islets as well as functional improvement.

关 键 词：C—MET 胰腺发育 RT—PCR WESTERN BLOT 

分 类 号：Q954.58[生物学—动物学]