金黄色葡萄球菌特异性卵黄抗体的制备及其影响因素  

Production of Staphylococcus Aureus-specific IgY and Its Influencing Factors

作　　者：甄宇红[1,2] 金礼吉[2] 关兴杰[1] 舒晓宏[1] 李晓宇[2] 王宇[1] 徐永平[2] 

机构地区：[1]大连医科大学药学院,辽宁大连116044 [2]大连理工大学生物科学与生物技术系,辽宁大连116024

出　　处：《现代生物医学进展》2009年第1期53-55,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30371053);辽宁省教育厅高等学校科学研究项目(2005121)

摘　　要：目的:考察金黄色葡萄球菌特异性卵黄抗体制备过程中的影响因素。方法:以灭活的金黄色葡萄球菌为抗原,采用不同的浓度(108cfu/mL,109cfu/mL,1010cfu/mL)、在不同免疫佐剂(商品弗氏佐剂和自制弗氏佐剂)作用下,对不同日龄(120天和300天)的蛋鸡进行免疫。免疫后收集鸡蛋,蛋黄用6倍体积水稀释,采用ELISA法测定抗体效价。结果:抗原浓度为109cfu/mL所得抗体效价最高;免疫120日龄蛋鸡获得抗体效价高于免疫300日龄蛋鸡所得抗体效价;在抗原浓度和蛋鸡日龄相同的情况下,商品弗氏佐剂比自制具有更好的免疫增强作用。结论:特异性卵黄抗体制备受多种因素的影响,抗原浓度、蛋鸡日龄、佐剂质量均对卵黄中特异性抗体水平有影响。Objective： To explore the influencing factors of production of Staphylococcus aureus-specific IgY. Methods： Laying hens in different age （120-day old and 300-day old） were immunized with formaldehyde killed Staphylococcus aureus at various concen- trations （10^8, 10^9, 10^10 cfu/ml） using different adjuvant （commercial Freund＇s adjuvant and self-prepared Freund＇s adjuvant）. The collected yolks were diluted by water at a ratio of 1：6, and the titer of water soluble fractions was tested by ELISA. Results： The optimal antigen concentration was 10^9 cfu/ml. The titer of IgY from 120-day old hens was higher than that from 300-day old hens. The immunization enhancement of commercial Freund＇s adjuvant was stronger than that of prepared Freund＇s adjuvant. Conclusions： The level of specific IgY in yolks of immunized hens was affected by concentration of antigen, age of hens and quality of adjuvant.

关 键 词：卵黄抗体(IgY) 抗原 佐剂 蛋鸡 

分 类 号：Q75[生物学—分子生物学] Q813