两种大气压等离子体处理大肠杆菌的实验研究  

Sterilization of Escherichia Coli with Two Atmospheric Pressure Plasmas

作　　者：王淑敏[1] 张贵新[1] 解云飞[1] 冯剑[1,2] 张庆[1] 

机构地区：[1]清华大学电机工程与应用电子技术系,北京100084 [2]河北省石家庄市供电公司,石家庄050021

出　　处：《高电压技术》2009年第1期42-47,共6页High Voltage Engineering

基　　金：国家自然科学基金(50477005)~~

摘　　要：为验证等离子体对大肠杆菌的杀灭效果并探讨其灭菌机理,进行了大气压下同轴介质阻挡放电射流装置和APMTP+JET型微波等离子体源两种等离子体发生装置激发等离子体的灭菌实验,并由活菌计数分别得出大肠杆菌存活曲线,通过扫描电镜对比灭菌实验前后大肠杆菌的状态,以对大气压等离子体灭菌机理进行初步分析。实验证明,该微波等离子体灭菌效果远好于同轴介质阻挡放电等离子体射流;两种装置对大肠杆菌的杀灭过程呈不同变化趋势;推测灭菌作用的主导因素为活性基团和高能粒子,其中静电电荷积累有一定作用。对于APMTP+JET装置微波等离子体,热效应不可忽略,其他因素则协同灭菌。To test and verify the results of bacteria sterilization with plasma and to analyze the inactivation mechanisms, we get results of a series of experimentations by E. coli treated with two plasmas-DBD plasma jet and APMPS＋JET plasma. We obtain the life eurves of E. coli（Escherichia coli）by taking account of the bacteria nurtured in medium after being treated, and compare SEM （scanning electron microscopy ） photographs taken before and after experimentation. It is found that the microwave plasma works better than DBD plasma jet does; the life curves show different regulations when treated by different plasma; active base and high-energy nuclear physies probably play an important role in experiments, and accumulated electrostatic charge takes effect; for microwave plasma, the high-temperature can not be ignored; other factors work in coordination.

关 键 词：大气压 等离子体 大肠杆菌 灭菌 机理 微波 

分 类 号：Q93-3[生物学—微生物学]