基于生物信息学与生物技术开发植物分子标记的研究进展  

The Progress of Development for Plant Molecular Markers Based on Bioinformatics and Biotechnology

作　　者：张征锋[1] 肖本泽[2] 

机构地区：[1]华中师范大学遗传调控与整合生物学湖北省重点实验室,武汉430079 [2]华中农业大学植物科学与技术学院,武汉430070

出　　处：《分子植物育种》2009年第1期130-136,共7页Molecular Plant Breeding

基　　金：国家自然科学基金(30800680)资助

摘　　要：各种序列数据库的迅猛增长与生物信息软件的不断开发使基于序列资源的标记开发更加便捷,各类分子生物学技术的涌现及高通量检测平台的建设为实验室开发分子标记提供了强有力的技术支持。本文从生物信息学角度概述了SNP、SSR等分子标记开发的方法、特点及应用现状,并介绍了利用分子生物技术开发SSR、SRAP、TRAP标记的手段及从RAPD、AFLP等标记向单位点特异性PCR标记(如SCAR、CAPS、dCAPS等)转化的进展。最后,对不同植物标记开发策略的选择及以上各种技术在功能标记开发中的应用前景作了展望。The genome sequencing and expressed sequenced tag （EST） projects generated a vast amount of sequences data. Simultaneously, the increasing bioinformatics software facilitates the development of molecular markers. Additionally, to develop molecular markers in laboratory will benefit from the advanced biotechnology and construction of batch of operation systems. The methods, characteristics and corresponding application of developing SNP and SSR based on bioinformatics and developing SSR, STAP, TRAP on biotechnology were reviewed. The progress of conversion of RAPD and AFLP to single-locus specific PCR markers were also presented. Finally, how to select suitable technique for different plants and develop functional markers are elucidated.

关 键 词：标记开发 生物信息学 生物技术 功能标记 

分 类 号：S336[农业科学—作物遗传育种] Q943[农业科学—农艺学]