VIGS:植物功能基因组学研究的革命  

VIGS: the Revolution of Plant Function Genomics Research

作　　者：姚丹青[1] 张微微[1] 原丽华[1] 潘俊松[1] 何欢乐[1] 蔡润[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《分子植物育种》2009年第1期155-161,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(30671111);上海市重点学科建设项目(B209)资助

摘　　要：病毒诱导基因沉默(virus induced gene silencing,VIGS)技术是一种RNA介导的抗病毒防御反应机制,目前在植物反向遗传学领域已经表现出巨大的潜力。VIGS技术不仅优于传统的植物转基因技术,方法简便,高效耐用,而且具有高通量特性。在功能基因组学领域的研究中,这些优越性已经使VIGS技术成为最具吸引力的首选技术手段。目前,VIGS体系应用最成功的植物是病毒学家常用的模式植物-本氏烟草(Nicotiana benthamiana),与此同时,也在努力改良VIGS技术,使其能够在包括单子叶植物在内的其它物种中得到广泛应用。我们讨论的重点是针对利用VIGS技术来确定基因功能,该技术已经在抗病性,逆境胁迫,细胞信号传导以及次生代谢生物合成途径研究中显示出相关基因功能的多样性,并随之构建出一系列新的相关模型。Virus-induced gene silencing （VIGS） is a technology that exploits an RNA-mediated antiviral defense mechanism and has been shown to be of great potential in plant reverse genetics. Because of the methodological simplicity, robustness, and speedy results than plant normal transformation, VIGS has been used widely for analysis of gene function and high-throughput functional genomics in plant. Until now most applications of VIGS have been in Nicotiana benthamiana. Moreover, new vector systems and methods are developed to use in other plants, including monocots. Here, we discuss the issues specific to the application of VIGS technology to determine gene function, which has revealed the roles of a variety of genes in disease resistance, abiotic stress, cellular signaling and secondary metabolite biosynthesis and then construct a series of new related models.

关 键 词：VIGS 病毒载体 基因沉默 功能基因组学 

分 类 号：Q943[生物学—植物学]