七叶皂苷体外抗SGC-7901细胞的作用及机制研究  

Studies of the mechanism and inhibitory effect of aescine on SGC-7901 cells in vitro

作　　者：吴子侠[1] 吴海竞[1] 曹莉[1] 孙晓飞[1] 明志君[1] 张学光[2] 

机构地区：[1]苏州大学医学部药学院,江苏苏州215123 [2]苏州大学医学生物技术研究所,江苏苏州215123

出　　处：《中国药理学通报》2009年第1期78-81,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30330540);苏州市"三药"科技攻关资助项目(NoN3132702);苏州大学博士学位人员资助项目(No13132743)

摘　　要：目的研究七叶皂苷体外抗SGC-7901肿瘤细胞的作用及其机制。方法采用MTT法观察药物对SGC-7901肿瘤细胞的增殖抑制作用;采用流式细胞仪检测SGC-7901肿瘤细胞周期的变化;FITC-AnnexinⅤ/PI双标记检测SGC-7901肿瘤细胞的凋亡;Western blot检测凋亡相关蛋白Bcl-2的表达。结果七叶皂苷可明显抑制SGC-7901肿瘤细胞的增殖,并抑制细胞周期和诱导细胞发生凋亡,可下调凋亡相关蛋白Bcl-2的表达。结论七叶皂苷体外对SGC-7901肿瘤细胞具有良好的抑制作用,并可诱导细胞发生凋亡。Aim To study the mechanism and inhibitory effect of Aescine on SGC - 7901 cells in vitro . Methods MTT method was used to detect SGC-7901 proliferation. Flow cytometer method was used to deter- mine cell cycles. Annexin V-FITC apoptosis derection kit was used to detect apoptosis of cells. Western blot was employed to analyze the expression of Bcl-2. Resuits MTT resuh showed that Aescine could signifi- cantly inhibit the growth of SGC-7901. Annexin VFITC apoptosis derection kit and Western blot results suggested that Aescine could induce the apoptusis of SGC-7901. Conclusions Aescine can inhibit the growth of SGC-7901 and tn vitro. induce the apoptosis of cells in vitro.

关 键 词：七叶皂苷 细胞凋亡 SGC-7901肿瘤细胞 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]