bFGF特异性结合噬菌体的活性研究  

Activity Study of Specific bFGF-Binding Phage

作　　者：颜秋霞[1,2] 吴晓萍[3] 黄慧贤[2] 聂昌君[2] 聂燕芳[3] 柯实[2] 肖健[1] 李校堃[1] 

机构地区：[1]温州医学院药学院/浙江省生物技术制药工程重点实验室,温州325035 [2]暨南大学医药生物技术研究开发中心,广州510632 [3]暨南大学组织移植与免疫实验中心,广州510632

出　　处：《中国生物工程杂志》2009年第1期23-26,共4页China Biotechnology

基　　金：浙江省自然科学基金重点项目(Z205755);浙江省卫生高层次创新人才培养工程(李校堃);教育部跨新世纪优秀人才计划(NCET-05-0537)资助项目

摘　　要：研究从噬菌体随机7肽库中筛选的bFGF特异性结合噬菌体的活性。采用ELISA检测3轮淘选后得到的阳性噬菌体克隆对bFGF的结合曲线。通过竞争抑制实验,分析bFGF结合噬菌体与bFGF的竞争结合活性。采用MTT法检测bFGF结合噬菌体对由bFGF诱导的Balb/c3T3细胞增殖的影响。从噬菌体肽库中淘选获得的阳性噬菌体克隆能够特异地与bFGF结合,并呈剂量依赖性,其与固相bFGF的结合能被游离的bFGF竞争抑制,并可抑制由bFGF诱导的Balb/c3T3细胞增殖。bFGF特异性噬菌体可拮抗bFGF的活性,为开发针对bFGF的抗肿瘤短肽类新药提供基础。To study the activity of a specific bFGF-binding phage selected from a phage display heptapeptide library. EL1SA was applied to assess the bFGF-binding curve of the positive phage clone obtained from 3 rounds screening. The competitive binding activity of the bFGF- binding phage was examined by competitive inhibition assay. The effect of the bFGF-binding phage on the proliferation of Balb/c 3T3 cells induced by bFGF was investigated by M3T method. The positive phage selected from the phage library specifically band to bFGF in a dose-dependent manner, and its binding with the immobilized bFGF was competitively inhibited by free bFGF. The isolated phage could also inhibit the proliferation of Balb/c 3T3 cells induced by bFGF. The bFGF-binding phage could inhibit bFGF activity, which would offer the basis for further developing the anti-tumor short peptide targeting bFGF.

关 键 词：噬菌体展示肽库 碱性成纤维细胞生长因子 bFGF结合噬菌体 

分 类 号：Q71[生物学—分子生物学]