龟鹿二仙胶汤含药血清对体外软骨细胞表型BMSCs凋亡的影响  

Effects of Guilu Erxian Decoction Serum on Apoptosis of Chondrogenic Phenotype Bone Marrow-Derived Mesenchymal Stem Cells in Vitro

作　　者：李文顺[1] 李楠[2] 王和鸣[2] 李西海[2] 詹红生[1] 石印玉[1] 汤亭亭[3] 戴克戎[3] 李月平 王永强[5] 张林[6] 

机构地区：[1]上海中医药大学曙光医院骨伤科,上海200021 [2]福建中医学院骨伤系 [3]上海交通大学医学院第九人民医院骨科 [4]山东省东营市胜采医院 [5]山东中医药高等专科学校骨伤系 [6]复旦大学药学院

出　　处：《中国中医骨伤科杂志》2009年第1期5-7,共3页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：国家自然科学基金课题(No.30772814);教育部博士学科点专项基金课题(No.20050393002);福建省教育厅科研课题(No.JA04212)

摘　　要：目的:观察龟鹿二仙胶汤含药血清干预软骨细胞表型化骨髓质基质干细胞(BMSCs)凋亡的影响。方法:抽取兔骨髓液,经体外分离、纯化、培养获得免BMSCs,将龟鹿二仙胶汤含药血清诱导出的软骨细胞表型化BMSCs,用IL-1β诱导凋亡,分别于24h、48h、72h后,采用透射电子显微镜、免疫荧光组织化学、流式细胞仪等实验技术,研究龟鹿二仙胶汤对软骨表型化BMSCs凋亡的影响。结果:龟鹿二仙胶汤能减少软骨表型化BMSCs凋亡的数量及程度,降低细胞凋亡率。结论:龟鹿二仙胶汤可以明显抑制或逆转软骨表型化BMSCs凋亡,能显著延缓软骨表型化BMSCs的凋亡。Objective：To observe the effect of Guilu Erxian decoction （GED） serum on the apoptosis of chondrocyte pheno- type differentiation of bone marrow--derived mesenchymal cells （BMSCs）. Methods：The BMSCs were isolated from rabbit bone marrow, and the cells were extracted, purified and cultured in vitro. The cells that show chondrogenie phenotype differentiation of BMSCs were induced into apoptosis cells by IL-1β. after 24, 48 and 72 hours, transmission electron microscope, immune histochemistry, flow cytometry analysis were used to observe the effect on chondrocyte phenotype of BM- SCs apoptosis. Results..GED decreased quantity and degree of apoptosis in Chondrogenic Phenotype cells and reduced the apoptosis of BMSCs. Conclusion：GED can significantly prevent or reverse the apoptosis of chondrogenic phenotype and delay the apoptosis of chondrogenie phenotype.

关 键 词：BMSCS TGF-Β3 诱导分化 龟鹿二仙胶汤 IL-1β 凋亡 

分 类 号：R683.1[医药卫生—骨科学]