双环醇对小鼠日本血吸虫病心肌组织中TGF-β_1、金属蛋白酶1和Ⅰ、Ⅲ型胶原表达的影响  

Effect of bicyclol on the expression of myocardial TGF-β_1,TIMP_1 in mice infected with Schistosoma japonicum

作　　者：何生松[1] 徐标[1] 韩春荣[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院感染科,武汉430022

出　　处：《中国人兽共患病学报》2009年第1期34-37,41,共5页Chinese Journal of Zoonoses

摘　　要：目的研究小鼠日本血吸虫病肝纤维化致心肌损伤作用及双环醇对心肌损伤及纤维化的保护效应及机制。方法80只小鼠随机均分为4组。其中3组小鼠感染日本血吸虫8周后,分别以双环醇60mg/(kg·d)、120mg/(kg·d)治疗56d(8周)以及不作任何治疗(实验对照组),第4组小鼠作为健康对照组。应用苏木素伊红(HE)染色及免疫组化染色法,观察并分析不同剂量双环醇治疗前后各组小鼠心肌组织病理改变、转化生长因子β1(TGF-β1)、金属蛋白酶组织抑制因子(TIMP1)和Ⅰ、Ⅲ型胶原的表达变化同步观察肝组织病理改变。结果用高剂量双环醇治疗后,小鼠心肌组织、肝组织病理损伤减轻,心肌组织中TGF-β1、TIMP1和Ⅰ、Ⅲ型胶原含量明显低于实验对照组而高于健康对照组,而低剂量双环醇对日本血吸虫病致肝纤维化及心肌损伤无明显治疗效果。结论日本血吸虫病可致心肌损伤。双环醇抗日本血吸虫病肝纤维化、保护心肌作用呈剂量依赖性,通过抑制心肌组织中TGF-β1、TIMP1和Ⅰ、Ⅲ型胶原过度表达减轻心肌纤维化程度,从而保护心肌作用。To study the effects and mechanisms of myocardial injury in mice with hepatic fibrosis inducted by Schistosoma japonicum infection, and to investigate the protective effects and mechanisms of bicyclol on the myocardial injury. 80 mice were divided into four groups： in which three groups were infected with Schistosoma japonicum. Eight weeks after infection, two group among the three groups was treated with bicyclol 60 mg/（kg· d） and 120 mg/（kg· d） for 8 weeks respectively and the third was taken as experimental control without any treatment. The fourth group was taken as normal control. HE staining, electric microscope and immunohistochemical technique were applied to observe the pathological changes in liver tissue and myocardium of mice, the expressions of myocardial transforming growth factorβ1 （TGF-β1）, tissue inhibitor of metalloproteinase： （TIMP1）, type Ⅰ and type Ⅲ collagen were examined before and after treatment with bicyclol . It was demonstrafed that high dosage of bicyclol treatment significantly relieved the degree of hepatic fibrosis and myocardium injury, reduced the contents of hepatic TGF-β1, TIMP1, type Ⅰ and type Ⅲ collagen compared to the experimental control. There is no difference on therapeutical effects between the group of low dosage of bicyclol treatment and experimental control. It is concluded that hepatic fibrosis due to advanced schistosomiasis may lead to myocardial injury. The effect of bicyclol on liver fibrosis and myocardial injury induced by Schistosoma japonicum infection is determined by its dosage. Bicyclol may protect myocardium through relieving the degree of hepatic fibrosis and inhibiting the overexpression of myocardial TGF-β1, TIMP1 and Ⅰ , Ⅲ type collagen.

关 键 词：双环醇 日本血吸虫病 肝纤维化 心肌损伤TGF-β1 TIMP1 

分 类 号：R532.2[医药卫生—内科学]