中外两品种鸡胸肌组织差异表达基因的研究  

Screening and identification of differentially expressed genes in Beijing fatty and broiler breast muscles

作　　者：冯浩咏[1,2] 苗向阳[1] 王希彪[2] 胡婕[1,2] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]东北农业大学动物科技学院,哈尔滨150030

出　　处：《遗传》2009年第1期83-87,共5页Hereditas（Beijing)

基　　金：国家高技术研究发展计划(863计划)资助项目(编号：2008AA10Z140);“十一五”国家科技支撑计划资助项目(编号：2006BDA13B08);中国农业科学院北京畜牧兽医研究所科技创新团队资助项目(编号：ywf-td-1)

摘　　要：运用mRNA差异显示技术对北京油鸡和AA肉鸡胸肌组织基因的差异表达进行研究,从分子水平分析导致两品种肌肉组织基因差异表达的机制。通过反向Northern dot blot技术验证共筛选出差异表达基因7条,经与GenBank数据库进行相似性比对,S1与HMGN3基因有较高同源性:S3与ChEST294a8有很高同源性,但功能未知;S4和S5与鸡的磷酸葡萄糖变位酶Ⅰ同源性很高;S6和S2与已有核酸数据库中的基因克隆或EST具有较高同源性,为已知的EST,但功能未知;序列S7未在数据中发现同源序列,可以确定其在AA肉鸡中特异表达,确定为新发现的EST(GenBank登录号:EU594549)。为进一步研究中外两品种鸡胸肌组织基因差异表达机制奠定了基础。mRNA differential display reverse-transcripton PCR（DDRT-PCR） was applied to identify differentially expressed genes in Arbor Acres broiler（AA） and Beijing fatty chicken breast muscles in order to find the mechanism which induces the differential gene expression at the molecular level. A total of 7 ESTs were found using reverse Northern dot blot, and all of them were compared with the nucleotide sequences in GenBank database using BLAST. S1 was highly similar to HMGN3; S3 was highly similar to ChEST294a8 with unknown functions; S4 and S5 were highly similar to PGM Ⅰ ; S6 and S2 had highly similar nucleotide sequences with unknown functions in nucleotide databases; S7 had no significant similar- ity with existing genes or ESTs and was regarded as a new EST. The new EST was submitted to GenBank（Accession number： EU594549）. This lays a foundation for further study on the mechanism of differential gene expression in Beijing fatty and AA breast muscles.

关 键 词：鸡 MRNA差异显示 胸肌 

分 类 号：S831[农业科学—畜牧学]