超声破坏微泡联合G-CSF促进心肌梗死大鼠血管新生  

Ultrasound-targeted microbubble destruction combined with granulocyte colony-stimulating factor facilitates angiogenesis of infarcted myocardium in rats

作　　者：薛静[1] 王志刚[1] 许川山[1] 张群霞[1] 任建丽[1] 冉海涛[1] 

机构地区：[1]重庆医科大学超声影像学研究所,重庆400010

出　　处：《第四军医大学学报》2009年第1期29-32,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金重点项目(30430230);国家"863"科技攻关项目(2006AA02Z4F0)

摘　　要：目的:探讨超声破坏微泡联合粒细胞集落刺激因子(G-CSF)刺激大鼠梗死心肌血管内皮生长因子(VEGF)分泌、促进血管新生的有效性.方法:心肌梗死模型Wistar大鼠40只,随机分为:超声+微泡造影剂+G-CSF(A组);超声+微泡造影剂组(B组);单纯G-CSF组(C组);单纯手术(SA)组(D组).于治疗后2wk处死大鼠取材,采用免疫组化法检测心肌组织CD34的表达,并在显微镜下计数心肌内新生血管密度(MVD).采用酶联免疫吸附法(ELISA)检测心肌组织内VEGF蛋白表达情况.结果:免疫组化显示A组心肌组织中有大量CD34表达,新生血管最多,MVD计数为(231±24)个/高倍镜视野,明显高于B组(148±19),C组(100±12),D组(86±8)个/高倍镜视野,差异具有统计学意义(P<0.05).ELISA检测结果显示A组心肌组织中VEGF蛋白表达量为(3.14±0.21)ng/g,明显高于B组(2.56±0.17),C组(1.91±0.14),D组(0.97±0.11)ng/g,差异具有统计学意义(P<0.05).结论:超声破坏微泡可刺激缺血心肌内源性VEGF分泌,促进心肌血管新生,联合G-CSF能明显增强其血管新生作用.AIM： To explore whether ultrasound-targeted microbubble destruction （US/MB） combined with granulocyte colonystimulating factor （ G-CSF ） can facilitate secretion of vascular endothelial growth factor （VEGF） and angiogenesis of the infarcted myocardium in rats. METHODS： Forty myocardial infarction Wistar rats models were randomly divided into 4 groups： US/MB ＋ G-CSF treatment group （A）; US/MB alone treatment group （B）; G-CSF alone treatment group （C）; and surgery alone （SA） group （D）. Two weeks later, the rats were killed and the myocardia were harvested. The CD34 expression was detected by immunohistochemistry, and microvessel density （MVD） was counted in high-power field （HPF） under microscope. The VEGF expression in myocardium was detected by ELISA. RESULTS： There was significant CD34 expression in the myocardium of group A, and the MVD was higher in group A [ （231 ±24）/HPF] than in group B[（148±19）/HPF], C[ （100 ±12）/HPF] and [（86±8）/HPF1 （P 〈0.05）. The content of VEGF in the myocardium of group A[ （3.14 ± 0.21 ） ng/g] was higher than those in groupB[（2.56±0.17）ng/g], C[（1.91±0.14）ng/g ], and D[ （0.97 ± 0.11 ） ng/g] （P 〈 0.05）. CONCLUSION： US/MB can facilitate the endogenous secretion of VEGF and accelerate the vascularization to the infarcted myocardium in rats. Pretreatment with G-CSF can augment the angiogenic effect of US/MB.

关 键 词：超声微泡 粒细胞集落刺激因子 血管新生 

分 类 号：R445.1[医药卫生—影像医学与核医学]