CYP11B2-344T/C基因多态性与2型糖尿病肾病的关系  

Correlation between CYP11B2-344T/C gene polymorphism and diabetic nephropathy in patients with type 2 diabetes mellitus

作　　者：张晓梅[1] 孙卫华[1] 徐浣白[1] 周纯先[1] 石建华[1] 

机构地区：[1]蚌埠医学院第一附属医院内分泌科,233004

出　　处：《实用医学杂志》2009年第1期56-59,共4页The Journal of Practical Medicine

基　　金：安徽省卫生厅基金资助项目(编号:2002A040)

摘　　要：目的:研究2型糖尿病(T2DM)患者CYP11B2-344T/C基因多态性与糖尿病肾病(DN)的相关性以及与DN不同分期的关系。方法:将145例T2DM患者分为DN组73例和糖尿病非肾病组(NDN组)72例,另选择52例门诊体验健康人为正常对照组(NC组)。应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)对以上197例观察对象进行基因型分析。结果:(1)DN患者存在CYP11B2-344T/C多态性,本研究197例观察对象中CYP11B2-344T/C多态性存在CC、CT、TT3种基因型,频率分别为12.7%、50.3%、37.1%;C、T等位基因频率分别为31.2%、68.8%。(2)DN组CYP11B2-344T/CT等位基因频率明显高于NC组(P<0.05)。(3)DN组CYP11B2-344T/C多态性CC、CT、TT3种基因型各临床指标均数比较差异无显著性(均P>0.05)。(4)DN组内微量白蛋白尿期、临床白蛋白尿期和肾功能不全期CC、TT、CT基因型频率和C、T等位基因频率差异均无显著性(均P>0.05)。(5)CYP11B2-344T/C基因型与DN无相关性(P>0.05)。(6)DN患者TT基因型血浆醛固酮水平最高。结论:CYP11B2-344T/C多态性与2型糖尿病肾病无显著相关性。Objective To explore the association between CYP11B2-344T/C gene polymorphism and diabetic nephropathy. Methods 145 patients with type 2 diabetes whose genotypes were analyzed by polymerase chain reactionrestriction fragment length polymorphism（PCR-RFLP） were divided into diabetic nephropathy group （group DN, n = 73） and non-diabetic nephropathy group （group NDN, n = 72）, another 52 normal controls as control group（group NC）. Results There were CC（12.7%）, CT（50.3%）, and TT（37.1%） genotypes of CYP11B2-344T/C in the 197 objects. The frequencies of genotype C and T allele were 31.2% and 68.8%, respectively. The frequency of CYP11B2- 344T/C T allele in group DN was significantly higher than that in group NC （P 〈 0.05）. There were no significant differences in the clinical indexes of CC, CT and TT genotypes of CYP11B2-344T/C, so were the frequencies of CC, TT, CT genotypes and C, T allele in different stages of diabetic nephropathy in group DN（P 〉 0.05）. There was no significant correlation between CYP11B2-344T/C genotype and diabetic nephropathy（P 〉 0.05）. The plasma aldosterone level was the highest in TT genotype in group DN. Conclusion There is no significant correlation between CYP11B2- 344T/C genotype polymorphism and diabetic nephropathy in patients with type 2 diabetes mellitus.

关 键 词：2型糖尿病 糖尿病肾病 醛固酮合酶 多态现象 遗传 CYP11B2-344T/C基因 多态性 

分 类 号：R587.2[医药卫生—内分泌] R544.1[医药卫生—内科学]