虎源猫泛白细胞减少症病毒VP1、Vp2和NS1基因的克隆与序列分析  

Cloning and Sequence Analysis of VP1,VP2 and NS1 Genes of Feline Panleukopenia Virus Isolated from Tiger

作　　者：于亚丽[1] 华育平[1] 曾祥伟[1] 田丽红[1] 夏咸柱[2] 刘丹[3] 

机构地区：[1]东北林业大学,黑龙江哈尔滨150040 [2]军事医学科学院军事兽医研究所 [3]黑龙江东北虎林园

出　　处：《东北林业大学学报》2009年第1期83-85,共3页Journal of Northeast Forestry University

基　　金：国家林业局野生动植物保护管理项目资助

摘　　要：根据Genbank上发表的猫泛白细胞减少症基因序列数据，设计了3对引物，并采用PCR方法对从东北虎粪便中分离出的FPV-HLJ的结构蛋白VP1、VP2和非结构蛋白NS1基因进行了扩增，将各片段克隆至pMD18-T载体，经PCR鉴定后进行了序列测定和分析。结果显示：FPV-HU与GenBank上公布的FPV、CPV和MEV毒株相比，核苷酸序列同源率VP1为98.8％-99.8％，VP2为98.1％-99.4％，NS1为98.6％-99.7％。VP1氨基酸同源率为97.6％-99.2％，VP2、NS1氨基酸同源率与其核苷酸同源率相同。并且VP1、VP2和NS1上分别有4、3和9处氨基酸发生变异。Based on the sequences of the VP1, VP2 and NS1 genes of Feline Panleukopenia Virus（FPV） published in Gen-Bank, three pairs of primers were＇designed to amplify the VP1, VP2 and NS1 genes of FPV-HLJ strain isolated from tiger feces. The genes were amplified by polymerase chain reaction（ PCR）, and subsequently cloned into pMD18-T vector. Resuits of sequence analysis showed that the nucleotide homologies between FPV-HLJ and the published sequences were 98.8%-99.8% for VP1,98.1% - 99.4% for VP2, 98.6% - 99.7% for NS1. The amino acid homologies was 97.6%-99.2% for VP1, and the amino acid homologies were the same as the nucleotide homologies for VP2 and NS1. Four, three and nine amino acid variations were found in VP1, VP2 and NS1, respectively.

关 键 词：猫泛白细胞减少症病毒 VP1 VP2 NS1 基因 克隆 序列分析 

分 类 号：S852.652[农业科学—基础兽医学] S858.99[农业科学—兽医学]