同步辐射X射线小角散射法研究纳米铁材料在生物介质中的粒度分布  

Particle size distribution of iron nanomaterials in biological medium by SR-SAXS method

作　　者：王云[1,2] 荆隆[1] 丰伟悦[1] 汪冰[1] 王华建[1,2] 朱墨桃[1,2] 王萌[1] 欧阳宏[1] 赵宇亮[1] 柴之芳[1] 吴忠华[3] 

机构地区：[1]中国科学院高能物理研究所核分析技术重点实验室及纳米生物效应与安全性联合实验室,北京100049 [2]中国科学院研究生院,北京100049 [3]中国科学院高能物理研究所同步辐射国家实验室,北京100049

出　　处：《核技术》2009年第1期1-5,共5页Nuclear Techniques

基　　金：国家重大基础研究专项基金(2006CB705605);中国科学院和国家自然科学基金(10490180;20475055;10675139)资助

摘　　要：在研究纳米材料的生物效应时,首先需要对纳米材料在生物介质中的物理化学性质进行全面详细的表征。受生物介质中高蛋白浓度和高离子强度的影响,纳米颗粒在生物介质中极易团聚,对纳米颗粒在生物分散体系中的粒度表征十分困难。本实验采用同步辐射X射线小角散射法(SR-SAXS)研究纳米Fe、Fe2O3和Fe3O4颗粒及其在磷酸盐缓冲液(PBS)、DMEM培养基分散体系中的粒度分布,并与TEM结果进行比较。结果显示SR-SAXS可准确测试纳米Fe、Fe2O3和Fe3O4颗粒的粒度分布,且不受分散体系和分散浓度的影响。表明SR-SAXS可很好地应用于测试稳定性较差的非均匀分散体系中纳米颗粒的粒度分析。A better understanding of biological effects of nanomaterials in organisms requests knowledge of the physicochemical properties of nanomaterials in biological systems. Affected by high concentration salts and proteins in biological medium, nanoparticles are much easy to agglomerate, hence the difficulties in characterizing size distribution of the nanomaterials in biological medium. In this work, synchrotron radiation small angle X-ray scattering （SR-SAXS） was used to determine size distributions of Fe, Fe2O3 and Fe3O4 nanoparticles of various concentrations in PBS and DMEM culture medium. The results show that size distributions of the nanomaterials could perfectly analyzed by SR-SAXS. The SR-SAXS data were not affected by the particle content and types of the dispersion medium. It is concluded that SR-SAXS can be used for size measurement of nanomaterials in unstable dispersion systems.

关 键 词：X射线小角散射 纳米铁材料 分散体系 粒度表征 

分 类 号：O433[机械工程—光学工程] O648.14[理学—光学]