b型流感嗜血杆菌荚膜多糖纯化方法的优化  

Optimization on Purification of Capsular Polysaccharide from Haemophilus influenzae Type b

作　　者：尹珊珊[1] 李贵凡[2] 刘学龙[1] 郑海发[2] 

机构地区：[1]延边大学农学院,吉林龙井133400 [2]北京民海生物科技有限公司,北京102600

出　　处：《动物医学进展》2009年第1期50-52,共3页Progress In Veterinary Medicine

摘　　要：对b型流感嗜血杆菌荚膜多糖纯化工艺中冷酚抽提次数、沉淀核酸的乙醇浓度、沉淀糖精的乙醇浓度进行研究。结果表明,最佳纯化工艺为冷酚抽提4次,300 mL/L的乙醇沉淀核酸,650 mL/L的乙醇沉淀糖。所建立的提取纯化工艺提取的荚膜多糖纯度高,各项指标能够达到WHO的规程要求。Cool penaol extraction times, ethanol concentration for precipitating nucleic acid and polysaecharide of purification conditions were studied in the article. The results showed that cold phenol extracting 4 times, 300 mL/L ehanol precipitating nucleic acid and 650 mL/L ethanol precipitating polysaccharide, respectively, were the optimal conditions, suitable for purification of Haemophilus influenzae type b capsular polysaccharide. The capsular polysaccharide purified accroding to the above conditions had high purity, all the indexes reached WHO requirement.

关 键 词：B型流感嗜血杆菌 荚膜多糖 纯化 

分 类 号：Q939.122[生物学—微生物学] Q539