白花蛇舌草对肝癌细胞移植瘤HSP70及P16表达的影响  

Effect of Herba Hedyotis Diffusae on Expression of Heat Shock Protein70 and P16 in Mice with Hepatoma Cell-transplanted Tumor

作　　者：胡玲[1] 古学文[1] 唐纯志[1] 谢宇 崔娜娟 罗晓韵[1] 

机构地区：[1]广州中医药大学,广州510405 [2]北京市中西医结合医院,北京100039

出　　处：《中药新药与临床药理》2009年第1期18-20,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省“千百十工程”优秀人才基金(Q02026)。

摘　　要：目的观察白花蛇舌草对H22肝癌细胞移植瘤组织HSP70及P16抑癌基因蛋白表达的影响。方法将经中药处理后的H22肝癌细胞免疫小鼠,以HSP70单抗封闭后分为白花蛇舌草未封闭、封闭组,党参未封闭、封闭组及RPMI-1640空白对照组,共5组,然后再以培养的H22肝癌细胞攻击小鼠形成移植瘤。免疫组化标记法检测各组小鼠移植瘤组织HSP70及P16抑癌基因蛋白的表达。结果白花蛇舌草及党参未封闭组HSP70蛋白的表达上调,尤以白花蛇舌草组上调较为明显,与两药封闭组及空白对照组之间比较,具有显著性差异。白花蛇舌草及党参未封闭组、封闭组P16抑癌基因蛋白的表达均上调,与空白对照组比较,差异均具有显著性;白花蛇舌草上调P16抑癌基因蛋白的作用强于党参,但两药未封闭与封闭组之间比较,并无显著性差异。结论白花蛇舌草可能是通过诱导H22肝癌细胞移植瘤组织HSP70的表达,提高其免疫原性而达到抗肿瘤的作用。白花蛇舌草及党参上调P16抑癌基因蛋白表达的作用似乎与HSP70的诱导没有直接联系。Objective To ohselare the effect of Herba Hedyotis Diffusae （HHD） on expression of heat shock protein 70 （HSP70） and P16 anti - oncogene protein in mice with H22 cell transplanted tumor. Methods The mice were immunized with hepatoma - lines - H22 cells which were treated by Chinese herbs firstly. According to the pretreatment of the Chinese herbs and whether the H22 cells being blocked by HSP70 monoclonal antibody, the mice were divided into 5 groups ： HHD non - blocking group, HHD - blocking group, Radix Codonopsis （RC） - blocking group, RC non - blocking group, and RPMI - 1640 control group. Then the treated H22 Cells were translated into the mice. The expression of HSP70 and P16 anti - oneogene protein in mice with transplanted H22 liver carcinoma was detected by immunohistochem- ical marking method. Results The expressions of HSP70 was up - regulated in HDD non - blocking group and RC non - blocking group, in particular in RC non - blocking group, and the difference was significant as compared with HHD - blocking group, RC -blocking group and control blank group. The expression of P16 anti -oneogene protein was upregulated in the treatment groups, and the difference was significant in comparison with the control group. The effect on P16 anti -oncogene protein expression was stronger in HDD groups than that in RC groups, but the difference was insignificant between the blocking group and the non - blocking group of each herb. Conclusion The anti - tumor effect of HDD may be achieved by inducing HSP70 expression in transplanted H22 neoplasia and by enhancing the immunogenicity. The up -regulation of P16 anti -oncogene expression by HHD and RC has no direct relationship with the induction of HSP70, and the related mechanism needs further research.

关 键 词：白花蛇舌草 H22肝癌细胞 热休克蛋白70 P16抑癌基因 

分 类 号：R285.5[医药卫生—中药学]