HPLC法测定五指毛桃中补骨脂素和芹菜素的含量  

Determination of Psoralen and Apigenin in Radix Fici Hirtae by HPLC

作　　者：李明[1] 陈志维[1] 黎玉翠[1] 陈振权 陈楚镇 苏子仁[1] 

机构地区：[1]广州中医药大学,广州510006 [2]河源市金源绿色生命有限公司,广东河源517000

出　　处：《中药新药与临床药理》2009年第1期52-54,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立五指毛桃中补骨脂素和芹菜素的含量测定方法。方法采用高效液相色谱法对五指毛桃药材中补骨脂素和芹菜素进行含量测定。色谱柱:YMC C18柱(250mm×4.60mm,5μm);流动相:甲醇-0.2%磷酸,梯度洗脱;流速:1.0mL·min-1;柱温:30℃;检测波长:补骨脂素245nm,芹菜素338nm;进样量:10μL。结果补骨脂素在0.0836～1.0450μg范围内线性关系良好,r=0.9999,平均回收率100.43%,RSD%=0.45%(n=9);芹菜素在0.0656～0.8200μg范围内线性关系良好,r=0.9999,平均回收率100.41%,RSD%=0.34%(n=9)。结论所建立的方法简便、快捷,重现性好,可用于该药材的质量评价。Objective To establish the quantitative methods of psoralen and apigenin in Radix Fici Hirtae. Methods The content of psoralen and apigenin in Radix fici Hirtae was determined by HPLC. The chromatographic conditions were as follows： YMC C18 column （250 mm×4.60 ram, 5μm）, mobile phases of methanol -0. 2 % phosphoric acid for gradient elution, flow rate being 1.0 mL · min^-1, column temperature at 30℃, detection wavelength being 245 nm for psoralen and 338 nm for apigenin, and inject volume being 10 μL. Results Psoralen showed a good linear relationship in the range of O. 083 6 - 1. 045 0μg, r = 0. 999 9, the average recovery was 100. 43 % and RSD % was 0.45 %. Apigenin showed a good linear relationship in the range of 0. 065 6 - 0. 820 0μg, r = 0. 999 9, the average recovery was 100.41% and RSD % was 0. 34 %. Conclusion The established methods are simple and rapid with good reproducibility, and can be used for the quality control of Radix fici hertae.

关 键 词：五指毛桃 补骨脂素 芹菜素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]