RP-HPLC法测定消渴通络胶囊中芍药苷的含量  

Determination of Paeoniflorin in Xiaoke Tongluo Capsule by RP-HPLC

作　　者：林荣锋[1] 何耀慧[1] 黄晓其[1] 陈剑平[1] 赖小平[1] 苏子仁[1] 

机构地区：[1]广州中医药大学新药研究开发中心,广州510006

出　　处：《中药新药与临床药理》2009年第1期61-63,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立消渴通络胶囊中芍药苷的含量测定方法。方法采用高效液相色谱法测定消渴通络胶囊中芍药苷含量。色谱柱:Phenomenex Luna C18柱(4.6mm×250mm,5μm);流动相:乙腈-0.1%磷酸梯度洗脱;柱温30℃;检测波长:230nm;进样量:10μL。结果芍药苷在0.298～2.984μg范围内与峰面积呈良好线性关系,r=0.9999;芍药苷平均回收率为100.45%,RSD=1.80%(n=9)。结论该定量方法简便可行、重复性好,可用于消渴通络胶囊的质量控制。Objective To establish a RP - HPLC method for determining paeoniflorin in Xiaoke Tongluo Capsule. Methods The content of paeoniflorin in Xiaoke Tongluo Capsule was determined by RP - HPLC. Phenomenex Luna C18 column （4.6 mm×25 0mm, 5 μm） was used, with a mobile phase of A （acetonitrile） - B（0. 1% phosphoric acid）, and with a gradient elution. The flow rate was 1.0 mL · min^-1, the column temperature was 30 ℃, the detection wavelength was 230 nm, and the injection volumn was 10 μL. Results The linear range of paeoniflorin was 0. 298 - 2. 984μg, r = 0. 999 9. The average recovery of paeoniflorin was 100.45 % （RSD = 1.80 % , n = 2）. Conclusion The quantitative method is simple, feasible and reproducible, and can be used for quality control of Xiaoke Tongluo Capsule.

关 键 词：消渴通络胶囊 芍药苷 RP—HPLC法 含量测定 

分 类 号：R284.1[医药卫生—中药学]