绵羊TTF-1基因分子克隆及序列分析  

Molecular Cloning and Sequence Analysis of Ovine TTF-1 Gene

作　　者：韩芬霞[1] 张利平[1] 吴建平[1] 万红玲[2] 李丽娟[1] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]天水师范学院,甘肃天水741000

出　　处：《湖南农业科学》2009年第1期131-133,共3页Hunan Agricultural Sciences

基　　金：甘肃省自然科学基金项目(3ZS061-A25-074)

摘　　要：以经产小尾寒羊母羊基因组DNA为模板,利用特异性引物对P1和P2对绵羊甲状腺转录因子-1(TTF-1)基因进行扩增,克隆入pGEM-T载体,转化后挑取阳性克隆进行酶切与测序鉴定,并对获得的TTF-1基因序列及推导的氨基酸序列进行生物信息学分析。结果表明,扩增的绵羊TTF-1基因序列长度为1459bp,包括部分外显子1、外显子2序列以及内含子,共编码174个氨基酸。该基因与GeneBank报道牛、狗、猪、人、和小鼠的基因序列同源性分别为98.7%、98.3%、97.5%、96.6%和96.6%,内含子与猪、人、鼠的内含子同源性为83.7%、74.9%和59.5%。Taking total DNAs from blood of Small Tail Han sheep as template, ovine thyroid transcription factor 1 （TTF-1） was amplified by specific primers （P1 and P2）. Then pGEM-T vector was cloned into, positive clones were screened and digested by restriction endonuclease after transforming, and TTF-1 gene sequence and its deduced amino acid sequence were analyzed. The results showed that the sequence length of the TTF-1 gene was 1 459 bp, encoded for 174 amino acids including parts of Exon 1 and Exon 2 seguences and one intron. The exon sequences of ITF-1 gene shared 98.7% ,98.3% ,97.5%,96.6% ,96.6% homology with the published genomic sequences of dog, pig, cattle, human and mouse. And the intron sequences of TTF-1 gene shared 83.7%, 74.9%, 59.5% homology with the published genomic sequences of pig, human and mouse.

关 键 词：绵羊 甲状腺转录因子-1基因 分子克隆 序列分析 

分 类 号：S826[农业科学—畜牧学] Q78[农业科学—畜牧兽医]