穴位埋线对慢性应激雌性大鼠下丘脑-垂体-性腺轴的影响  

Influence of Acupoint Catgut-embedding Therapy on the Hypothalamus-pituitary-gonad Axis in Female Chronic Stress Rats

作　　者：徐朝霞[1] 张宏[1] 谭少明[1] 边心会[1] 范春[1] 

机构地区：[1]广州中医药大学,广东510405

出　　处：《上海针灸杂志》2009年第1期54-56,共3页Shanghai Journal of Acupuncture and Moxibustion

基　　金：广东省中医药管理局课题(1060079)

摘　　要：目的探讨穴位埋线对慢性应激模型大鼠下丘脑-垂体-性腺轴(HPG轴)的调节作用。方法采用制动应激方式作用于雌性SD大鼠21天,制成慢性应激模型,在百会、内关、三阴交、太冲穴进行穴位埋线,每5天1次,共埋线4次。运用放射免疫法(RIA)检测大鼠下丘脑GnRH、血清LH、E2和Cor的含量,并与对照组、模型组做比较。结果模型组大鼠下丘脑GnRH、血清LH和血清E2的含量明显低于对照组(P<0.05);埋线组下丘脑GnRH、血清LH和血清E2的含量明显高于模型组(P<0.05),与对照组比较差异无统计学意义(P>0.05)。模型组大鼠血清Cor含量明显高于对照组(P<0.05);埋线组血清中Cor含量明显低于模型组(P<0.01),与对照组比较差异无统计学意义(P>0.05)。结论慢性应激引起雌性大鼠HPG轴受到抑制,穴位埋线可以减轻应激对HPG轴的抑制作用。Objective To investigate the regulative effect of acupoint catgut-embedding therapy on the hypothalamus-pituitary-gonad axis in a rat model of chronic stress. Methods Female SD rats were treated by immobilization stress for 21 days to make a model of chronic stress. Catgut was embedded at points Baihui（GV20）, neiguan（PC6）, sanyinjiao（SP6） and taichong（LR3） once every five days for 4 times. Rat hypothalamic GnRH and serum LH, E2 and Cor contents were measured by radioimmunoassay. A comparison was made with the control group and the model group. Results Hypothalamic GnRH and serum LH and E2 contents were significantly lower in the model group than in the control group （P〈0.05）. Hypothalamic GnRH and serum LH and E2 contents in the acupoint catgut-embedding group were significantly higher than those in the model group （P〈0.05） and not significantly different from those in the normal group （P〉0.05）. Serum Cor content was significantly higher in the model group than in the normal group （P〈0.05）. Serum Cor content in the acupoint catgut-embedding group was significantly lower than those in the model group （P〈0.01） and not significantly different from those in the normal group （P〉0.05）. Conclusion Chronic stress inhibits the HPG axis in female rats and acupoint catgut-embedding therapy can reduce the inhibitory effect of stress on the HPG axis.

关 键 词：应激 埋线 下丘脑-垂体-性腺轴 糖皮质激素 

分 类 号：R2-03[医药卫生—中医学]