He-Ne激光诱变原生质体选育四环素高产菌的研究  

LASER MUTATION ON PROTOPLASTS AND THE BREEDING OF TETRACYCLINE HIGH-YIELDING STRAIN

作　　者：陈五岭[1] 罗雯[1] 姚胜利[2] 

机构地区：[1]西北大学生物学系,西安710069 [2]中国科学院西安光学精密机械研究所,西安710068

出　　处：《光子学报》1998年第4期330-333,共4页Acta Photonica Sinica

基　　金：陕西省自然科学基金

摘　　要：采用He－Ne激光对金霉素链霉菌（S．aureofaciens）原生质体诱变处理，结果表明：He－Ne激光对该菌原生质体具有明显的致死作用．经激光处理的原生质体再生株产抗生素能力与亲本菌株相比，四环素产量平均提高9．32％，最高提高20．6％．Protoplasts of streptomyces aureofaclens were irradiated by He-Ne laser,the result showed that He - Ne laser had obvious lethal effect on protoplasts. The unit of tetracycline fermentation of regenerate strains after irradiating was tested. Compared with the controlled one,it increased yield by about 9. 32% on average,the highest one increased by about 20. 6%.

关 键 词：激光诱变 金霉素链霉菌 原生质体 诱变 四环素 

分 类 号：TQ465.92[化学工程—制药化工] Q939.13[生物学—微生物学]