低聚核苷酸d(GGTATACC)_2的~1H和^(13)CNMR研究  

1H AND 13 C NMR STUDY OF DEOXYOLIGONUCLEOTIDE d(GGTATACC) 2 IN SOLUTION

作　　者：阎江丽[1,2] 张友杰[1,2] 袁汉珍[1,2] 毛希安[1,2] 沈联芳[1,2] 

机构地区：[1]中国科学院武汉物理与数学研究所波谱与原子分子物理国家重点实验室 [2]华中师范大学分析测试中心

出　　处：《波谱学杂志》1998年第2期117-122,共6页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金

摘　　要：通过综合分析２Ｄ１ＨＮＯＥ谱（混合时间１００ｍｓ），１３Ｃ－１Ｈ异核多量子化学位移相关谱和１Ｈ双量子滤波化学位移相关谱归属了低聚脱氧核糖核酸片断ｄ（ＧＧＴＡＴＡＣＣ）２１Ｈ和１３Ｃ的化学位移．该８聚核苷酸在溶液中为双螺旋Ｂ型结构，各碱基相对于糖环呈反式构象，糖环本身则均以Ｃ２′－ｅｎｄｏ构象为主．Deoxyoligonucleotide d(GGTATACC) 2 was studied by two dimensional NMR techniques. Its proton and 13 C chemical shifts were assigned by 2D proton NOE spectrum and 13 C- 1H heteronuclear multiquantum chemical shift correlated spectrum (HMQC) combined with double quantum filtered chemical shift correlated spectrum (DQF-COSY). The sugar puckers of the eight residues of this helix octamer were determined to be dominantly C2′-endo forms with anti glycosidic conformations.

关 键 词：化学位移 核苷酸 NMR 低聚核苷酸 氢1 碳13 

分 类 号：Q524.01[生物学—生物化学]