复方蒲黄颗粒对局灶性脑缺血再灌注大鼠神经元凋亡及Caspase-3 mRNA表达的影响  

Effects of Puhuang Granules on Neuronal Apoptosis and Caspase-3 mRNA Expression in Rats Following Cerebral Ischemia-reperfusion

作　　者：夏韵[1] 向军[2] 刘小雨[1] 石红军[3] 

机构地区：[1]同济大学附属第十人民医院中医科,上海市200072 [2]复旦大学附属中山医院中医科,上海市200032 [3]同济大学分子生物学实验室,上海市200092

出　　处：《中国药房》2009年第3期168-170,共3页China Pharmacy

基　　金：上海市科学技术委员会科研计划项目(064119515)

摘　　要：目的:观察复方蒲黄颗粒(PH)对局灶性脑缺血再灌注大鼠神经元凋亡的影响,并探讨其作用机制。方法:采用大脑中动脉线栓法建立局灶性脑缺血再灌注大鼠模型。75只大鼠分为假手术、模型和PH高、中、低剂量组。采用Nissl染色观察神经元形态变化,TUNEL法检测神经元凋亡,原位杂交检测Caspase-3mRNA的表达。结果:PH高、中剂量组少量细胞核固缩深染,细胞丢失、凋亡细胞数及Caspase-3mRNA阳性细胞数均显著减少。结论:PH可下调Caspase-3的表达,从而抑制神经元凋亡,对脑缺血再灌注损伤有保护作用。OBJECTIVE： To investigate the protective effects of Compound Puhuang granules（PH） on neural apoptosis in rats with focal cerebral ischemia reperfusion, and to discuss the possible mechanism of its neuron protection.METHODS： The rats were surgically prepared for occlusion of the middle cerebral artery＇ （MCAO） according to the technique of Zea - Longa et al .Totally 75 SD rats were randomized into 5 groups（n = 15）： sham operation（Sham） group; ischemia/ reperfusion（I/ R） group;PH of high dose （PHH） group; PH of medium does（PHM） group;PH of low dose（PHL） group. The morphologic changes of neurons were observed by using Nissl staining; the neuron apoptosis was detected by TUNEL method and the expression of Caspase - 3 mRNA were examined by using in situ hybridization.RESULTS： In PHH and PHM groups, karyopyknosis anachromasis was noted in small percentage of cells, and the cell loss, the number of apoptotic cells and the number of Caspase- 3 mRNA positive cells in the two groups were all significantly reduced.CONCLUSION：PH could inhibit neural apoptosis in rats with focal cerebral ischemia - reperfusion through down - regulating the expression of Caspase - 3 mRNA so as to achieve a protective effect on cerebral ischemia reperfusion injury.

关 键 词：复方蒲黄颗粒 脑缺血再灌注损伤 凋亡 CASPASE-3 

分 类 号：R285.5[医药卫生—中药学] R971[医药卫生—中医学]