PRV、PCV-2、PPV多重PCR检测方法的建立及其应用  

Establishment and application of a multiplex PCR for Pseudorabies virus,porcine circovirusⅡand porcine parvoviruses

作　　者：曲光刚[1] 沈志强[1,2] 管宇[1,2] 王金良[1] 肖跃强[1] 孙园园[3] 

机构地区：[1]山东省滨州畜牧兽医研究院,山东滨州256600 [2]山东绿都生物科技有限公司,山东滨州256600 [3]镇江出入境检验检疫局,江苏镇江212000

出　　处：《中国动物检疫》2009年第1期23-25,共3页China Animal Health Inspection

摘　　要：根据GenBank上已发表的猪伪狂犬病病毒(PRV)、猪Ⅱ型圆环病毒(PCV-2)、猪细小病毒(PPV)核苷酸序列,设计并合成能分别特异性扩增PRV、PCV-2、PPV的引物。经条件优化,建立了同时检测PRV、PCV-2、PPV的多重PCR方法,所扩增特异性产物片段大小分别为217bp、394bp、748bp。该方法不仅特异性强、敏感性高,还克服了对上述病原分别进行检测所带来的诸多弊端,为猪伪狂犬病、猪Ⅱ型圆环病、猪细小病的临床诊断和流行病学调查等研究提供了新手段。According to the nucleotide sequences of Pseudorabies virus （PRV）, porcine circovirus Ⅱ （PCV-2） and porcine parvoviruses（PPV）in GenBank,three pairs of primers were designed and synthesized.After the indexes of PCR were optimized, a multiplex PCR based on these three pairs of primers was established that specifically amplified the fragements of PRV,PCV-2 and PPV 217bp,394bp and 748bp in length respectively. The results indicated that the optimized multiplex PCR is both sensitive and specific, providing a new method for the clinical diagnosis and epidemiological research of the three diseases

关 键 词：多重PCR 猪伪狂犬病病毒 猪圆环病毒 猪细小病毒 

分 类 号：S852.659.1[农业科学—基础兽医学]