猪肿瘤抑制基因候选5的克隆与序列分析  

cDNA Cloning and Sequence Analysis of the Gene of Porcine Tumor Suppressor Candidate 5

作　　者：张志强[1] 杨国宇[1] 王伟杰[1] 韩立强[1] 王静[1] 武宇晓[1] 臧猛[1] 台玉磊[1] 

机构地区：[1]河南农业大学动物生理生化实验室,河南郑州450002

出　　处：《河南农业科学》2009年第1期112-114,共3页Journal of Henan Agricultural Sciences

基　　金：河南省重点科技攻关项目(0523010500)

摘　　要：基于电子延伸序列,克隆了仔猪肿瘤抑制基因候选5(TUSC5),并初步分析其组织分布情况;获得了TUSC5基因的全长序列636bp,包括开放阅读框架(ORF)534bp,编码177个氨基酸。克隆的仔猪TUSC5基因与人、小鼠、大鼠的TUSC5基因序列的同源性分别为83.5%,82.4%,82.6%,推测的氨基酸序列同源性分别为71.2%,77.5%,78%,成功克隆了猪TUSC5基因。In this study, the coding sequence of porcine tumor suppressor candidate 5（TUSC5） were amplified based＇on the in silico sequence information. The result showed that the full length of TUSC5 was 636 bp and the open reading frame was 534 bp long. The predicted protein-coding sequence was 177 amino acid residues. Identity analysis showed that the nucleotide sequence of porcine TUSC5 shares 83.5%, 82.4% and 82.6% with that of human, mouse and rat, respectively. The deduced protein sequence shares 71.2%, 77.5%, and 78% homology with that of human, mouse and rat, respectively. All of these data indicates that the porcine TUSC5 gene was successfully cloned in the present study.

关 键 词：猪 TUSC5 克隆 序列分析 

分 类 号：S828[农业科学—畜牧学]