DCN与BGN在发育期小鼠牙髓牙本质复合体形成中的作用研究  

Expression of biglycan and decorin in the developing pulpo-dentinal complex of postnatal mouse

作　　者：唐开亮[1] 李纾[1,2] 姜淑敏[3] 王建[1] 高燕[4] 于兰[1] 

机构地区：[1]山东大学口腔医学院牙周病科,山东济南250012 [2]山东大学口腔医学院生物医学实验室 [3]山东省荣军总医院口腔科 [4]山东省滨州医学院附属医院口腔内科

出　　处：《临床口腔医学杂志》2009年第1期30-32,共3页Journal of Clinical Stomatology

摘　　要：目的:研究富含亮氨酸的蛋白多糖类物质:核心蛋白聚糖(DCN)及双糖蛋白聚糖(BGN)在小鼠牙髓牙本质复合体不同发育时期的分布特点,探讨其在牙髓牙本质复合体形成过程中的作用。方法:取出生后第5d、10d、15d、25d的BALB/c小鼠36只,引颈处死,解剖分离含下颌第一磨牙区的下颌骨,新鲜配制4%多聚甲醛固定24h,甲酸-甲酸钠复合脱钙液脱钙1～3周,石蜡包埋,近远中向5μm连续切片。免疫组化PV两步法,观察各发育时期第一磨牙牙本质、成牙本质细胞及牙髓细胞中DCN、BGN的组织学分布特点。结果:牙本质形成初期,BGN在前期牙本质与成牙本质细胞强阳性表达。随后,前期牙本质与成牙本质细胞染色开始减弱,而牙髓细胞阳性表达呈逐渐增强趋势。第5d,DCN在前期牙本质中强阳性表达,而在牙本质、成牙本质细胞中一直为阴性;第13d,牙髓细胞开始呈弱阳性表达,随着牙齿发育而逐渐增强。在观察期间,两者在前期牙本质中持续阳性表达,并且随着牙本质的发育成熟,其染色逐渐减低。结论:DCN与BGN在牙体组织和牙发育过程中特定阶段聚集在特定的部位,二者可能在细胞与细胞、细胞与基质相互作用中发挥特异作用并参与牙本质的矿化过程。Objective： To study the distribution and expression of decorin and biglycan in developing normal periodontal tissues, so as to understand its role in puplo-dentinal complex formation. Method： Thirty six BALB/c mice in different developing stages were killed and their bilateral mandibular first molars with surrounding alveolar bones and gingival tussues were taken out, Power VisionTM two steps immunohistochemical method with anti-decorin and anti-biglycan was used to detect the tissue distribution and cellular localization of decorin and biglycan. Result： At 5 day of age biglycan was strongly expressed in predentin and odontoblasts. With the development of pulpo-dentinal complex, the reduction of expression in predentin and odontoblasts was observed, But it gradually raised in pulp. Decorin was expressed in predentin,but negative in dentin and odontoblast. Biglycan and decorin were continually expressed throughout the period of investigation. Conclusion： It is proposed that decorin and biglycan accumulate at defined stages during dental tissue differentiation and development, they may play specific roles in cell-cell and cell-matrix interactions.

关 键 词：核心蛋白聚糖 双糖链蛋白聚糖 牙髓牙本质复合体 

分 类 号：Q813.1[生物学—生物工程]