基质细胞衍生因子受体4在肌损伤组织肌卫星细胞的表达  

EXPRESSION OF CXC CHEMOKINE RECEPTOR 4 IN MUSCLE SATELLITE CELLS OF MUSCLE INJURY TISSUES

作　　者：李小雷[1,2] 黄厚今[1] 周光前[3] 杨志明[3] 解慧琪[3] 邓力[3] 

机构地区：[1]遵义医学院预防医学教研室,贵州遵义563003 [2]连云港中医药高等职业技术学校 [3]四川大学华西医院生物治疗国家重点实验室.干细胞与组织工程研究室

出　　处：《中国修复重建外科杂志》2009年第1期26-29,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家高技术研究发展计划(863)资助项目(2001AA216011);2007年贵州省科技厅基金资助项目(C-331SOD)~~

摘　　要：目的探讨注射心脏毒素后肌损伤的肌卫星细胞中基质细胞衍生因子受体4(CXC chemokine receptor4,CXCR4)的表达情况,为进一步研究肌肉退行性疾病的分子学发病机制和治疗方法提供理论依据。方法取C57雄性小鼠12只,于左侧股四头肌局部注射心脏毒素(5μg/只),建立小鼠肌损伤模型;右侧股四头肌作为自身对照。于注射后1、4d及1、2、4、6周处死小鼠,分离两侧股四头肌,取材行HE染色、免疫组织化学染色及RT-PCR检测分析CXCR4表达情况。结果HE染色示肌肉组织从损伤修复到再生的全过程。免疫组织化学染色检测示注射心脏毒素后1、4d和1、2、4、6周,肌肉组织内CXCR4表达分别为1955.6±150.3、2223.2±264.3、2317.6±178.7、3066.5±269.6、1770.9±98.7和1505.7±107.1,与正常肌肉组织(640.3±124.0)比较,差异均有统计学意义(P<0.001)。RT-PCR检测显示,正常肌肉组织、注射心脏毒素后1、4d和1、2、4、6周肌肉组织内CXCR4mRNA表达分别为0.349±0.006、0.822±0.013、0.882±0.025、1.025±0.028、1.065±0.041、0.837±0.011和0.777±0.015;肌损伤后各时间点与正常肌肉组织比较,差异均有统计学意义(P<0.001)。结论CXCR4可能在肌肉损伤修复过程中起重要作用。Objective To observe the expressions of CXC chemokine receptor 4 （CXCR4） in muscle satellite cells in situ of normal and cardiotoxin-intoxicated muscle tissues so as to further investigate the molecular mechanism involving in muscle regeneration such as progressing muscular dystrophy （PMD） for seeking the way to cure muscle retrogression. Methods The muscle injured model of 12 C57 male mice was made by injecting cardiotoxin （5 btg per mouse） in left quadriceps femoris, their right quadriceps femoris was used as control without any injection. The histological, immunohistochemical analysis and RT-PCR were done to investigate the expression of CXCR4 in the quadriceps femoris in situ after 1 day, 4 days, 1 week, 2 weeks, 4 weeks and 6 weeks. Results HE staining results demonstrated that the muscle tissues experienced the process from muscle injury, repair to regeneration. The result of immunohistochemistry showed that the expressions of CXCR4 in injured muscle tissue were 1 955.6 ± 150.3, 2 223.2 ± 264.3, 2 317.6 ± 178.7, 3 066.5 ± 269.6, 1 770.9 ± 98.7 and 1 505.7 ± 107.1 at 1 day, 4 days, 1 week, 2 weeks, 4 weeks and 6 weeks after injection of cardiotoxin, there was significant difference when compared with normal muscle （640.3 ± 124.0, P 〈 0.001）. The RT-PCR showed that the expressions of CXCR4 mRNA in injured muscle tissue were 0.82.2 ± 0.013, 0.882 ± 0.025, 1.025 ± 0.028, 1.065 ± 0.041, 0.837 ± 0.011 and 0.777 ± 0.015 at 1 day, 4 days, 1 week, 2 weeks, 4 weeks and 6 weeks after injection of cardiotoxin, there was significant difference when compared with normal muscle （0.349 ± 0.006, P 〈 0.001）. Conclusion CXCR4 may be the critical protein in the process of muscle impairment and reparation.

关 键 词：基质细胞衍生因子受体4 肌卫星细胞 心脏毒素 肌损伤模型 小鼠 

分 类 号：R685[医药卫生—骨科学]