1，6-二磷酸果糖对油酸型急性呼吸窘迫综合征大鼠治疗作用的研究  

An Experimental Study of Therapeutic Effect of FDP on Oleic Acid-Induced ARDS in Rats

作　　者：金明根[1] 金春梅[1] 蔡春玉[1] 夏书香[1] 孟繁平[2] 金元哲[2] 

机构地区：[1]延边大学附属医院呼吸内科,吉林延吉133000 [2]延边大学基础医学院生理学与病理生理学教研室

出　　处：《天津医药》2009年第1期43-45,共3页Tianjin Medical Journal

基　　金：吴阶平医学基金会资助课题(项目编号:320.2415.06115)

摘　　要：目的:探讨1,6-二磷酸果糖(FDP)对油酸致大鼠急性呼吸窘迫综合征(ARDS)治疗作用的可能机制。方法:将30只健康的Wistar大鼠随机分为对照组、模型组和治疗组,由股静脉注入药物,对照组注入生理盐水2.1mL/kg,ARDS模型组注入油酸0.1mL/kg和生理盐水2.0mL/kg,FDP治疗组注入油酸0.1mL/kg和用生理盐水稀释的FDP400mg/kg(2.0mL/kg),3h后测定血清肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)浓度,肺组织Na+-K+-ATP酶(NKA)活性,计算左肺湿/干重(W/D)比值,行肺组织病理学观察。结果:模型组与对照组比较,左肺W/D、血清中TNF-α、IL-1β浓度明显升高(P<0.01),而肺组织中NKA活性明显降低(P<0.01),肺组织出现明显病理损害;治疗组与模型组比较W/D、TNF-α、IL-1β、NKA改变较小(P<0.05或P<0.01),其肺损伤轻于模型组。结论:FDP可通过抑制TNF-α、IL-1β升高,提高肺组织NKA活性,对实验性ARDS大鼠起一定的治疗作用。Objective： To study the therapeutic effects of fructose-1, 6-diphosphate （FDP） on acute respiratory distress syndrome （ARDS） in rats induced by oleic acid （OA）. Methods： Thirty healthy Wistar rats were randomly divided into three groups, control group, ARDS group and FDP-treated group. In control group normal saline （NS 2.1 mL/kg） was injected via femoral vein in rats. In ARDS group rats were induced ARDS by OA （0.1 mL/kg） and NS （2.0 mL/kg） injected via femoral vein. In FDP-treated group, OA （0.1 mL/kg） and FDP （400 mg/kg, diluted in 2.0 mL/kg of NS） solutions were injected via femoral vein respectively. After three hours, the contents of tumor necrosis factor-alpha （TNF-α）, interleukin 1 β（IL- 1β） in serum, the activity of Na＋-K＋-ATPase （NKA） in lung tissue and the index（wet weight/dry weight）of left lung tissue were measured, and pulmonary pathological changes were compared between the three groups. Results： Compared with control group, the lung index in ARDS group showed a significant increase, as well as the levels of TNF-α, IL-1β in serum （P 〈 0.01）. The activity of NKA in lung tissue showed a significant decrease （P 〈 0.01） and more typical pathological changes in lung tissue treated by OA. The changes in W/D, TNF-α, IL-1β, NKA, and pathological changes in FDP-treated group were less than those in ARDS group（P 〈 0.05 or P 〈 0.01 ）. Conclusion： The results indicated that FDP inhibited the increase of IL-1β and TNF-α in serum, also increased the activity of NKA in lung tissue. FDP showed therapeutic effects on OA-induced ARDS in Rats.

关 键 词：果糖-二磷酸酶 呼吸窘迫综合征 成人 油酸 疾病模型 动物 大鼠 WISTAR 

分 类 号：R563.8[医药卫生—呼吸系统] R-332[医药卫生—内科学]