乙肝乙脑麻疹多表位噬菌体微球疫苗的体液免疫应答  

Humoral Immune Response Induced by Phage Displayed Hepatitis B-Japanese Encephalitis-Measles Polytope Vaccine in Chitosan-based Microspheres

作　　者：兰芸[1] 胡凝珠[1] 胡云章[1] 程玉兴[1] 瞿素[1] 徐军[1] 舒晓明[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所疫苗研究室,昆明650118

出　　处：《中国生物制品学杂志》2009年第1期56-58,共3页Chinese Journal of Biologicals

基　　金：国家863计划资助项目(2006AA02Z4C0);云南省社会发展攻关项目(2006SG05)

摘　　要：目的探讨乙肝乙脑麻疹多表位噬菌体微球疫苗的体液免疫应答效果。方法用壳聚糖制备乙肝乙脑麻疹多表位噬菌体微球疫苗,经腹腔、皮下和鼻腔3种途径免疫小鼠,ELISA检测小鼠血清特异性IgG抗体水平。结果3种途径免疫的小鼠均能产生针对各表位的特异性IgG抗体,鼻腔免疫效果最好;多表位噬菌体微球疫苗的抗体水平明显高于多表位噬菌体疫苗。结论壳聚糖微球疫苗投递系统可以提高多表位噬菌体疫苗的体液免疫效果。鼻腔免疫是多表位噬菌体微球疫苗的最佳免疫途径。Objective To study the humoral immune response induced by phage displayed hepatitis B（HB）-Japanese encephalitis（JE）-measles polytope vaccine in chitosan-based microsphercs. Methods Phage displayed HB-JE-measles polytope vaccine was prepared into chitosan-based mierospheres and used for immunization of mice by intraperitoneal, subcutaneous and intranasal routes respectively. The specific lgG level was determined by ELISA. Results Specific IgG levels against various epitopes were induced with the phage displayed polytopc vaccine in chitosan-based microspheres by the 3 routes, which were significantly higher than those with the vaccine not in chitosan-based microspheres. However, the lgG level induced with the vaccine in chitosan-based microspheres by intranasal route was significantly higher than those by the other 2 routes. Conclusion Chitosan-based microsphere delivery system enhances the humoral immune response induced by phage displayed polytope vaccine, lntranasal vaccination is the optimal route to deliver the phage displayed polytope vaccine in chitosan-based microspheres.

关 键 词：多表位噬菌休疫苗 壳聚糖微球 免疫途径 体液免疫应答 

分 类 号：R392-33[医药卫生—免疫学]