牦牛瘤胃细菌中核糖核酸含量及其与细菌总氮比值的研究  

THE CONTENT OF RIBONUCLEIC ACID AND THERATIO OF RIBONUCLEIC ACID TO BACTERIANITROGEN IN THE BACTERIA MIXTURESEPARATED FROM THE YAK RUMEN

作　　者：韩兴泰[1] 胡令浩[1] 谢敖云 石成银 王青莉 

机构地区：[1]青海省畜牧兽医科学院

出　　处：《动物营养学报》1998年第2期35-39,共5页CHINESE JOURNAL OF ANIMAL NUTRITION

摘　　要：７头带有瘤胃瘘管的生长阉牦牛，在两个试验中分别给予不同的日粮以研究其瘤胃细菌中核糖核酸（ＲＮＡ，下同）含量及ＲＮＡ与细菌总氮（ＢＮ，下同）的比值（ＲＮＡ／ＢＮ）和核糖核酸氮（ＲＮＡ—Ｎ，下同）与ＢＮ的比值（ＲＮＡ—Ｎ／ＢＮ）。试验Ⅰ的４头牛分两期分别喂给粗蛋白（ＣＰ）８％和１２％的等能（９６ＭＪＭＥ／ｋｇＤＭ）日粮；试验Ⅱ的３头牛通过３×３拉丁方设计喂给蛋白饲料为菜籽饼、豌豆和蚕豆，精粗比为７：３，５：５和３：７的等能（９６ＭＪＭＥ／ｋｇＤＭ）、等氮（ＣＰ为１２％）日粮。结果表明，日粮精粗比与蛋白来源对细菌中ＲＮＡ含量及ＲＮＡ／ＢＮ和ＲＮＡ－Ｎ／ＢＮ均无明显影响，而蛋白水平对其有显著影响。当日粮ＣＰ为８％时，瘤胃细菌中ＲＮＡ与ＣＰ含量及ＲＮＡ／ＢＮ与ＲＮＡ－Ｎ／ＢＮ分别为１６９％、２８８％、３７，０３３；当日粮ＣＰ为１２％时，则上述４项指标依次为９８５％、３７１％、１６９、０１５。? Seven growing yaks fitted with rumen cannula were used in two trials to study the content of ribonucleic acid(RNA)and the ratios of RNA:bacteria nitrogen(BN)and RNA nitrogen(RNAN):BN in the rumen bacteria of the animalsIn trial Ⅰ,four animals were used and given two isocaloric(96 MJ ME/kgDM)diets containing 8% and 12% crude protein(CP)In trial Ⅱ,three animals were used in a 3×3 latin square design and given isocaloric(96MJ ME/kgDM)and isonitrogenous(12%CP)diets,in which meals of rape seed,garden pea and broad bean were used as main nitrogen sources and the dietary concentrate straw ratio were designed to be 7:3,5:5 and 3:7No significent influence of the dietary ratio and the nitrogen source on RNA content and on RNA/BN or RNAN/BN ratio was foundHowever,remarkable differences in trial I between the two diets were foundRNA,CP,RNA/BN and RNAN/BN in bacteria for 8% CP diet were 169%,288%,37 and 033 respectivelyCorresponding mean values for 12% CP diets including different daitary concentrate:straw ratio and different nitrogen sources were 985%,371%,169 and 015 respectively

关 键 词：牦牛 瘤胃细菌 核糖核酸 细菌氮 核糖核酸氮 

分 类 号：S823.85[农业科学—畜牧学]