吗替麦考酚酯和环孢素A对大鼠慢性移植肾病细胞保护基因表达的影响  

Effect of Mycophenolate Mofetil Versus Cyclosporine A on Cytoprotective Genes Expression in Rat Kidney Allografts Undergoing Chronic Allograft Nephropathy

作　　者：滕东海[1,2] 卢一平[1] 张雁钢[1] 辛宇鹏[1] 曹贵华[1] 李响[1] 王莉[1] 王佳[1] 

机构地区：[1]四川大学华西医院泌尿外科,四川成都610041 [2]四川省乐山市人民医院泌尿外科,四川乐山614000

出　　处：《华西医学》2009年第1期135-137,共3页West China Medical Journal

摘　　要：目的:研究吗替麦考酚酯(MMF)和环孢素A(CsA)对大鼠慢性移植肾病中细胞保护基因A20、HO-1、Bcl-2和Bcl-XL表达的影响。方法:分别采用雄性SD大鼠和Wistar大鼠作为供受体建立强化缺血/再灌注损伤慢性移植肾病模型。对受者依据所采用的免疫抑制剂方案分组:对照组、CsA组、MMF组。在移植术后的相同时间点处死大鼠,切取移植肾脏。采用荧光定量RT-PCR和免疫组织化学方法检测A20、HO-1、Bcl-2和Bcl-XL的表达及其在移植肾中的定位情况。结果:在大鼠慢性移植肾病模型中,能够检测到所有上述四种细胞保护基因的表达。A20主要表达于血管内皮细胞和浸润之淋巴细胞。HO-1主要表达于浸润之淋巴细胞,但在肾小管上皮细胞也有表达。Bcl-2和Bcl-XL则主要表达于肾小管上皮细胞。MMF组A20的表达显著高于CsA组和对照组(P<0.01)。HO-1,Bcl-2和Bcl-XL的表达在MMF组和CsA组间无显著性差异(P>0.05)。结论:MMF对A20表达的增强可能与MMF减轻大鼠慢性移植肾病病变的机制有关。Objective： To investigate whether there is difference between mycophenolate mofetil（MMF） and eyclospo- rine A（CsA）in the expression of cytoprotective genes A20, heine oxygenase（HO）-1,Bcl-2, and Bcl-XL in rat kidney allografts undergoing chronic allograft nephropathy（CAN）. Methods：Sprague-Dawley rat renal grafts were orthotopically transplanted into Wistar rats following the procedure of Kamada with our modification. The recipients were divided into three oral treatment groups：vehicle group,CsA group and MMF group. At the same times after transplantation,the rats were sacrificed to harvest the renal allografts. The expression of four eytoprotective genes, A20, HO-1, Bcl-2 ,and Bcl-XL were analyzed in these grafted kidneys by quantitative reverse transcriptase polymerase chain reaction （RT PCR） and immunohistochimistry. Results ： Four cytoprotective genes were all detected in rat kidney allografts undergoing CAN. The expression of A20 in grafted kidneys was significantly higher in MMF group than that in CsA group or vehicle group（P d0.01）. There was no significant difference between CsA and MMF groups in the expression of HO-1 ,Bcl-2 and Bcl-XL （P〉0.05）. Conclusion：MMF can improve the expression of A20 in rat kidney allografts undergoing CAN. The correla tion between MMF and A20 provide an explanation for the mechanism by which MMF ameliorates transplant arterioscle- rosis in an experimental animal model of chronic rejection.

关 键 词：慢性移植肾病 细胞保护基因 吗替麦考酚酯 A20 

分 类 号：R692.9[医药卫生—泌尿科学]