XRCC1基因多态性与宫颈癌危险性的研究  

The relationship between XRCC1 polymorphisms and the risk of cervical cancer in Jiangsu population

作　　者：蒋雯[1] 王美林[2] 张治中[2] 陈小军[2] 朱慧[2] 钱年风[2] 傅士龙[1] 张正东[2] 韩素萍[1] 

机构地区：[1]南京医科大学第一附属医院妇产科,江苏南京210029 [2]南京医科大学公共卫生学院,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2009年第1期1-6,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(30872084);江苏省省属高校自然科学重大基础研究项目(08KJA330001);教育部生殖医学重点实验室开放基金

摘　　要：目的:探讨XRCC1基因多态性与江苏人群宫颈癌易感性之间的关系。方法:采用基于医院的分子流行病学病例对照研究方法,选取436例经组织病理学确诊为宫颈癌的新发患者作为病例组和503例年龄(±5岁)、性别相匹配的非肿瘤者作为对照组;采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)方法对XRCC1启动子区-77T>C和外显子10区的Arg399Glu基因多态性进行基因分型,比较不同基因型携带者患宫颈癌的危险性;通过分层分析探讨初潮年龄、患者年龄及产次对罹患宫颈癌的影响。结果:与XRCC1-77TT相比,-77TC/CC基因型可减少罹患宫颈癌的危险性(OR=0.64,95%CI=0.48～0.86)。携带3～4个危险等位基因者比携带1～2个等位基因者患宫颈癌的危险性更大(OR=1.44,95%CI=1.01～2.04)。分层分析结果显示,年龄较大和产次较多且携带3～4个危险等位基因者罹患宫颈癌的危险性分别增加1.64倍(95%CI=1.02～2.64)和1.66倍(95%CI=1.01～2.72)。本研究未发现XRCC1Arg399Glu多态性与宫颈癌之间存在显著性相关。结论:XRCC1基因启动子-77T>C多态性显著降低江苏地区汉族人群罹患宫颈癌的危险性。Objective：To explore the association of XRCC1 gene single nucleotide polymorphisms and the risk of cervical cancer. Methods：In a hospital-based case-control study of 436 patients with newly, diagnosed cervical carcinoma and 503 cancer-free controls frequency-matched by age（±5 years）, we genotyped the -77T〉C and Arg399Glu polymorphisms of XRCC1 gene by the poly-rnerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method. We further analyzed the interaction of age, menarche age, and parity. Results： We found that the -77T〉C variant genotypes were associated with significantly decreased risk of cervical cancer adjusted odds ratio（OR） = 0.64; 95% confidence interval（CI）=0.48-0.86, compared with the wild-type homozygote. Moreover, individuals with 3-4 risk alleles increased the risk of cervical cancer accompanied with older age（ 〉 50 years）（OR=1.64, 95%CI=1.02-2.64） and parity （OR=1.66,95%CI=1.01-2.72）. By contrast, no significant association was observed between the Arg399Gln polymorphism and cervical cancer risk. Conclusion：Our finding indicated that the XRCC1-77 CC/CT genotype was significantly associated with a decreased risk of cervical cancer in Jiangsu population.

关 键 词：XRCCl基因 宫颈癌 基因多态性 分子流行病学 

分 类 号：Q786[生物学—分子生物学]