非小细胞肺癌endocan、FLK-1的表达与血管生成相关性研究  

The Expression of endocan and FLK-1 and their correlations with angiogenesis in non small cell lung cancer

作　　者：万锦平[1] 黄建安[1] 穆传勇[2] 刘皓[1] 鲁常青[1] 

机构地区：[1]常州市第一人民医院呼吸科,江苏常州213003 [2]苏州大学附属第一医院呼吸科,江苏苏州215000

出　　处：《南京医科大学学报（自然科学版）》2009年第1期28-31,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:研究内皮细胞特异分子-1(endothelial cell specific-1,endocan)、血管内皮细胞生长因子受体(KDR/FLK-1)在非小细胞肺癌(NSCLC)中的表达及其临床意义,同时探讨endocan、FLK-1与非小细胞肺癌血管生成的相关性。方法:应用免疫荧光技术检测60例手术切除的肺癌组织、21例癌旁肺组织(距癌组织5cm以上)中的endocan表达水平;免疫组化SP法检测FLK-1表达,并计数微血管密度(MVD)。结果:endocan、FLK-1的表达在NSCLC组织中明显高于癌旁组织(P均<0.05);endocan、FLK-1的表达与NSCLC的淋巴结转移、TNM分期正相关(P均<0.05),而与NSCLC的组织学分类无关(P均>0.05);在NSCLC中MVD、endocan、FLK-1阳性表达组明显高于二者阴性表达组(P均<0.05);endocan、FLK-1在NSCLC中的表达呈正相关(r=0.8889,P<0.05)。结论:endocan、FLK-1在NSCLC组织的血管发生、进展等病理过程有重要作用。Objective：To investigate the expression of endocan and FLK-1 in NSCLC, and to explore the correlation with angiogenesis and other clinical pathophysiological characteristics of NSCLC. Methods：The expression of endocan was detected by using immunofluorescence technique in 60 specimens of NSCLC and 21 specimens of adjacent non-cancerous tissues as control group. Im- munohistochemical S-P method was performed to estimate the expression of FLK-1 and MVD. Results：The endocan-positive rate was 78.33%（47/60） in NSCLC group and 14.29%（3/21） in control group. The positive rate of FLK-1 was 71.67%（43/60）in NSCLC group and 19.05%（4/21） in control group. There was a significant difference between them （X^2= 27.01,17.68; all P 〈 0.05）. In stage Ⅰ＋Ⅱ and Ⅲ＋Ⅳ of NSCLC, the positive rates of endocan were 67.64%（23/34） and 92.30%（24/26） while the positive rates of FLK-1 were 58.82%（20/34） and 88.46%（23/26）. The differences of endocan and FLK-1 expression between stage Ⅰ＋Ⅱ and Ⅲ＋Ⅳ were significant respectively （X^2=5.28, 6.37, all P 〈 0.05）. In the group with lymph node metastasis and the group without lymph node metastasis, the positive rates of endocan were 84.78%（39/46） and 57.14%（8/14）, the positive rates of FLK-1 were 80.43% （37/46） and 42.86%（6/14）. The differences of endocan and FLK-1 expression between the two groups were also significant respectively（X^2= 4.83,7.46,all P 〈 0.05）. The MVDs in the endocan-positive group and the endocan-negative group were 25.16±5.12 and 18.97±3.28 respectively, the difference was significant （t=4.52, P 〈 0.05）. The MVDs in the FLK-1-positive group and the FLK-1-negative group were 24.65±4.34 and 18.92±3.35 respectively, the difference was also significant（t= 5.23,P 〈 0.05）. There was positive correlation between the expressions of endocan and FLK-1 in NSCLC（r = 0.8889, P 〈 0.05）. However, The positive rates of endocan were 77.42%（24/31） in adenocareinoma, 80.00%（20/25�

关 键 词：非小细胞肺癌 内皮细胞特异分子-1 血管内皮细胞生长因子受体 微血管密度 

分 类 号：Q786[生物学—分子生物学]