哮喘患儿CD4^+CD25^+调节性T细胞及FOXP3的表达  

Role of CD4^+CD25^+ regulation cells and expressing of FOXP3 in the pathogenesis of children with asthma

作　　者：史彧[1] 褚莉莉[1] 张兰芳[1] 赵德育[1] 

机构地区：[1]南京医科大学附属南京儿童医院呼吸科,江苏南京210008

出　　处：《南京医科大学学报（自然科学版）》2009年第1期117-119,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金面上项目(07NMUM079)

摘　　要：目的:研究CD4+CD25+调节性T细胞在哮喘儿童外周血中的比例改变,并探讨其临床意义。方法:采用细胞内染色的流式细胞术检测急性发作期哮喘患儿外周血CD4+CD25+Treg及其调控基因FOXP3的表达,并与健康对照组进行比较。结果:急性发作期哮喘患儿外周血CD4和CD25双阳性细胞所占比例与健康对照组无明显差异(P>0.05),而CD4+CD25+FOXP3调节性T细胞在外周血中的比例明显低于健康对照组(P<0.01)。结论:哮喘患儿外周血CD4+CD25+FOXP3调节性T细胞在外周血中的比例明显减少,可能与哮喘的发病机制有关。Objective：To investigate the change of CD4^＋CD25^＋ T regulation cells in peripheral blood in children with asthma. Methods： CD4^＋CD25^＋FOXP3 T regulate cells in children with asthma were measured by intracellular 3-colour flow cytometry in peripheral blood cells in children with asthma. Results ：There were similar frequencies of CD4^＋CD25^＋ T cells in peripheral blood of asthma children and health control. However, the quantity of CD4^＋CD25^＋FOXP3 T regulate cells was decreased significantly in peripheral blood of patients with asthma in comparison with that of normal controls （P 〈 0.01 ）. Conclusion：The proportion of CD4^＋CD25^＋FOXP3 T regulate cells decreased in children with asthma, which might contribute to the pathological process of such disease.

关 键 词：CD4^+CD25^+调节性T细胞 FOXP3 哮喘 流式细胞术 

分 类 号：R725.6[医药卫生—儿科]