经流式细胞仪分选神经细胞技术方法的建立  

Establishment of Sorting Method on Neural Cells by Flow Cytometry

作　　者：王萍[1] 李润今[1] 高学文[1] 王伟[2] 

机构地区：[1]内蒙古自治区医院神经内科,内蒙古呼和浩特010017 [2]华中科技大学同济医学院附属同济医院神经内科,湖北武汉430030

出　　处：《内蒙古医学杂志》2008年第12期1416-1418,F0003,共4页Inner Mongolia Medical Journal

基　　金：国家自然科学基金资助项目(30230140);内蒙古自然科学基金资助项目(200711020918)

摘　　要：目的:探索建立一种分离在体模型中不同种类神经细胞的技术方法。方法:采用免疫荧光标记方法和流式细胞仪分选技术。结果:NeuN和GFAP双标显色可见,GFAP阳性的星形胶质细胞无NeuN表达,经过流式细胞仪分选可获得高纯度的星形胶质细胞和神经元,其阳性标记率分别为31.91%±4.67%和47.70%±6.30%。结论:该分选方法可以同时获得纯度高、数量多的不同种类的神经细胞。Objective：To explore a novel sorting method on different types of neural cells in vivo. Methods： Astroeytes and neurons was detected by immunofluorescence and flow cytometry （FCM）. Results： GFAP positive cells with no NeuN expression were stained by immunofluorescent technique;There were a high purificating rate of astrocytes and neurons by sorting of flow cytometry, and its percentages were 31.91% ±4.67 % and 47.70 % ±6.30 %. Conclusion： The sortying method established has a great quantity and high purificating rate of different types of neural cells at same times.

关 键 词：流式细胞分选 星形胶质细胞 神经元 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R446.39[医药卫生—基础医学]