商陆对阿霉素肾硬化大鼠肾小球细胞凋亡介导因子Bcl-2、Bax表达的影响  

The effect of phytolaccae on the expression of apoptotic mediators Bcl-2,Bax in glomeruli in adriamycin-induced glomerulosclerosis rats

作　　者：朱永俊[1,2] 张克非[2] 郭明好[1] 王颖[2] 

机构地区：[1]新乡医学院第一附属医院肾脏病科,河南卫辉453100 [2]遵义医院肾病科

出　　处：《医学信息（手术学分册）》2008年第11期967-970,共4页Medical Information Operations Sciences Fascicule

基　　金：贵州省卫生厅科学基金(D-184)资助

摘　　要：目的观察商陆水煎剂对阿霉素肾小球硬化大鼠肾小球细胞凋亡介导因子Bcl-2、Bax的表达的影响。方法采用单侧肾切除并阿霉素尾静脉注射的方法建立大鼠肾小球硬化模型。48只SD大鼠随机分为假手术组,模型组,商陆低剂量组、商陆高剂量组,分别给予生理盐水、生理盐水、商陆水煎剂(2g·kg-1.d-1)、商陆水煎剂(4g·kg-1.d-1)治疗,连续治疗93d。观察商陆水煎剂对肾小球硬化大鼠尿蛋白、血肌酐、尿素氮及肾脏组织形态学的影响,并用TUNEL法测定肾小球细胞凋亡,免疫组化法检测肾组织中Bcl-2、Bax的表达。结果与模型组比较,商陆高、低剂量组大鼠尿蛋白含量、血肌酐、尿素氮水平均有下降;但商陆高剂量组与模型组差异显著(P<0.01)。商陆高、低剂量组肾小球硬化指数、凋亡指数、促凋亡蛋白Bax的表达及Bax/Bcl-2的比值较模型组明显降低(P<0.01);抗凋亡蛋白Bcl-2的表达明显高于模型组(P<0.01)。结论商陆能调节阿霉素肾小球硬化大鼠肾小球Bcl-2、Bax的表达,下调Bax/Bcl-2的比值,减轻肾小球实质细胞的过度凋亡,延缓肾小球硬化进展。Objective To observe the effects of Pokeberry root decoction on the expression of apoptotic mediators Bcl - 2, Bax in glomeruli in adriamycin - induced glomerulosclerosis rats. Methods Forty - eight male SD rats were randomly divided into four groups ： sham - operated group, model group, high - dose Phytolaccae - treated groups, and low- dose Phytolaccae -treated group. The glomerulosclerosis rats model was established via uninephrectomy and twice injections with adriamycin 5 mg/（kg ·d）, 3 mg/（ kg· d） intravenously on the 7th and 28th day after uninephrectomy respectively. On the subsequent days the rats were respectively gastric - perfused treatment. The levels of 24 h urinary protein excretion and Scr, BUN and characteristic changes about renal tissue morphology damage were observed, apoptosis was examined by TUNEL method and the expression of bax,bcl -2 in glomeruli was detected by im- munohistochemistry at the same time. Results 1. At the end of experimentation, the levels of 24 h urinary protein excretion were decreased in treated groups , comparing with that in the model group（ P 〈 0.01 ）. The levels of Scr in two phytolaccae - treated groups and BUN in high - dosage Phytolaccae group were lower than that in model group（P 〈 0. 01 ）, the mean levels of BUN in low - dosage phytolaccae group were significantly lower than that in model group though low - dosage phytolaccae group was similar to model group. Renal tissue morphology damage in glomeruli, the apoptosis index and the expression of Bax, Bcl - 2 in decoction - treated groups were weaker than that in model group （P 〈 0.01 ）. Conclusions Pokeberry root decoction can decrease the expression of bax, bcl - 2 and over - apoptotic.

关 键 词：肾小球硬化 商陆水煎剂 细胞凋亡 BAX BCL-2 

分 类 号：R692[医药卫生—泌尿科学]