小麦矮腥黑粉菌PCR快速检疫鉴定方法  

RAPID METHOD OF PCR FOR DWARF BUNT IDENTIFICATION

作　　者：王念武[1] 周卫川[1] 郭琼霞[1] 

机构地区：[1]福建出入境检验检疫局技术中心,福建福州350002

出　　处：《武夷科学》2008年第1期69-72,共4页Wuyi Science Journal

基　　金：福建科技重大专项专题(2006NZ0002-1);福建科技厅自然科学基金项目(X0650003)

摘　　要：设计一对特异性引物,对小麦矮腥黑粉菌(TCK)及其近似种进行PCR扩增,结果表明TCK可以扩增出200bp清晰条带,而其近似种未出现条带,该方法可以作为TCK的PCR快速检疫鉴定方法。Dwarf bunt （Tilletia Controversa Kuhn, TCK） is one of the most dangerous quarantine wheat diseases in the world and hardest to control. In this paper, a pair of specific primers were designed and the PCR products showed that only the two TCK samples amplified the 200bp specific band ,the others were not any amplified quick identification of TCK

关 键 词：小麦矮腥黑粉菌 PCR 小麦印度腥黑穗病菌 禾草腥黑粉菌 小麦网腥黑粉病菌 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]