湖南稻曲病菌群体遗传多样性分析  

Analysis of genetic diversity of Ustilaginoidea virens in Hunan

作　　者：谭小平[1,2] 宋建伟[1] 刘二明[1] 刘年喜[2] 王金辉[2] 肖启明[1] 

机构地区：[1]湖南农业大学生物安全科学技术学院,湖南长沙410128 [2]湖南省植保植检站,湖南长沙410005

出　　处：《湖南农业大学学报（自然科学版）》2008年第6期694-697,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省重点科技攻关项目(05NK1005)

摘　　要：选用10个随机引物,利用RAPD技术,分析了来自湖南不同县(区)的53个稻曲病菌株的群体遗传结构.结果表明:以遗传相似系数0.78,将供试53个菌株划分为5个谱系,这5个谱系所含菌株数分别为42、7、2、1、1个,分别占总株数的79%、13%、4%、2%和2%,其谱系Ⅰ为优势谱系.由此说明湖南稻曲病菌遗传群体有分化,但分化不明显;来自同一地区和不同地区同一品种的菌株大多数聚集在同一类.Genomic fingerprints of 53 strains of U. virens from different counties （districts） in Hunan were analyzed by 10 RAPD primers. The results showed that all strains tested were divided into five genetic lineages（ Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ） at 0.78 level of the genetic similarity through the clustering analysis of UPGMA. The five genetic lineages separately contained numbers ostrains, 42, 7, 2, 1 and 1, in which they accounted for the rates of 79%, 13%, 4%, 2% and 2%, respectively and the lineage I was the dominant one with the rates of 79%. And it showed that the genetic diversity of U. virens in Hunan was not significant. Besides, most strains obtained from the same cultivar in same and the different regions were guroped in the same lineage.

关 键 词：稻曲病菌 随机扩增多态性DNA 遗传多样性 

分 类 号：S435.111.46[农业科学—农业昆虫与害虫防治]