卵浆内单精子注射术治疗阻塞性无精症的不育症  

EPIDIDYMAS SPERM ASPIRATION WITH INTRACYTOPLASMIC SPERM INJECTION PROVIDE FERTILITY FOR MEN WITH OBSTRUCTIVE AZOOSPERMIA

作　　者：李满[1] 庄广伦[1] 邓春华[1] 李蓉[1] 张敏芳[1] 王长希[1] 邓明芬[1] 

机构地区：[1]中山医科大学附属第一医院生殖医学研究中心

出　　处：《中山医科大学学报》1998年第2期131-134,共4页Academic Journal of Sun Yat-sen University of Medical Sciences

摘　　要：目的：评价附睾取精术结合卵浆内单精子注射技术治疗阻塞性无精症所致不育症的疗效。方法：对２４例精液常规检查无精症的男性不育患者之妻子，进行超排卵治疗３０个周期。获卵当日同时从患者阴囊行微切口附睾取精液，或经皮附睾穿刺抽吸术获得活动精子，进行卵浆内注射。精子准备方法可从常规的Ｍｉｎｉｐｅｒｃｏｌ法简化为作者自创的“Ｎ”字游出法。结果：３０个治疗周期共获卵３７８个，其中２９８个（占７８．８％）为减数分裂中期Ⅱ的卵子，可行显微精子注射，受精率和卵裂率分别为５５．７％和９４．６％；每周期平均移植的胚胎数３．６个。在所有治疗周期中共妊娠９例，其中流产１例，已分娩３例，继续妊娠５例，妊娠率为３０．０％／周期（９／３０）。结论：精路阻塞的无精症不育患者，只要获得活动精子，结合卵浆内单精子注射，仍然有机会获得妊娠。Objective:To observe whether epididymas sperm could be used to perform intracytoplasmic sperm injection (ICSI) for men with obstructive azoospermia. Methods:24 obstructive azoospermic males, 30 cycles, with their wives underwent controlled ovarian hyperstimulation, on the day of her oocytes retrieval we did open or percutaneous epididymas sperm aspiration and ICSI. Nshape sperm swimout founded by ourselves is one kind of sperm preparation method which can be an alternative to minipercoll.Results:We retrieved 378 oocytes from 30 cycles, among them 298 was MⅡ oocytes suitable for ICSI. The fertilization rate was 55.7%,and cleavage rate was 94.6%. 3.6 embryos were transfered per cycle, and the pregnancy rate was 30%(9/30). Among 9 pregnant cases, 1 case was aborted, 3 cases were delivered, and 5 cases were still ongoing. Conclusion: Infertility caused by obstructive azoospermia may be treated with ICSI using aspirated epididymas sperm.

关 键 词：少精液症 体外受精 男性不育 卵浆内注射 

分 类 号：R698.205[医药卫生—泌尿科学] R321－33[医药卫生—外科学]