胎兔手术技术操作基础  

Techniques in fetal rabbit surgery

作　　者：冯国平[1] 孙广慈[1] 杨欣[1] 张海明[1] 张正文 刘志飞[1] 

机构地区：[1]中国医学科学院中国协和医科大学整形外科医院,北京100041

出　　处：《中华整形烧伤外科杂志》1998年第3期186-188,共3页

基　　金：国家自然科学基金资助

摘　　要：目的建立简单有效的胎兔外科手术方法,使术后成活率达到80％以上。方法在胎兔创伤愈合的研究中,采用在硫喷妥钠静脉麻醉下将胎兔胎体后1/2置于宫外或在宫内手术的方法;用37℃左右的生理盐水补充术中丢失的羊水;严密缝合羊膜。结果手术后胎兔的成活率达到90％,胎兔发育正常。结论此方法简单有效。Objective Experimental fetal surgery has been a popular way to study growth and development and wound healing. The rabbit continues to be a reasonable animal model. The key to suc- cess of the experiment lies in keeping the survival rate of the operated animal above 80 percent. In this paper a simple experimental method for fetus surgery in rabbits is described. Methods Intravenous thiopental sodium is used for general anesthesia. Surgery is performed inside the uterus through hystero- tomy or with the caudal part of the fetus out of the uterus. Amniotic fluid is restituted by instillation of warm saline at 37℃. Special care is taken to suture the amnion. Results Using these techniques careful- ly, we have had 90 percent of success in fetal surgery experiment. Conclusion A technically simple pro- cedure has been developed for fetal rabbit surgery.

关 键 词：胎兔 外科手术 胎儿 

分 类 号：R726.2[医药卫生—儿科] R714.431[医药卫生—临床医学]