ox-LDL对人脑微血管内皮细胞活性及黏附分子表达的影响  

Influence of ox-LDL on activities of human brain microvascular endothelial cells and expressions of adhesion molecules

作　　者：张海艳[1] 朱陵群[1] 王硕仁[1] 张允岭[2] 

机构地区：[1]北京中医药大学东直门医院中医内科学教育部重点实验室,北京100700 [2]北京中医药大学东方医院

出　　处：《北京中医药大学学报》2008年第12期823-825,829,866,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2006CB504805)

摘　　要：目的观察氧化修饰低密度脂蛋白(ox-LDL)对体外培养人脑微血管内皮细胞活性及细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)表达的影响,探讨毒损脑络的现代分子生物学机制。方法以培养人脑微血管内皮细胞为靶细胞,在内皮细胞培养基中加入不同浓度的ox-LDL(0、5、10、25、50、75、100、150 mg/L)培养24、48、72 h,用四唑盐比色(MTT)法检测细胞活性,并以MTT测得的实验结果为基础选取ox-LDL浓度组(0、50、75、100 mg/L)和时间点(24、72 h),采用细胞免疫化学法和图像定量分析系统观察ICAM-1和VCAM-1的变化。结果ox-LDL对内皮细胞活性的影响有时间和浓度依赖性,统计学差异显著。细胞免疫化学法和图像定量分析系统显示ox-LDL各组(50、75、100 mg/L)OD值在24、72 h与正常对照组相比都有显著性差异(P(0.01),相同浓度组在24、72 h相比无统计学差异。结论不同浓度的ox-LDL能改变体外培养人脑微血管内皮细胞的活性,提高ICAM-1和VCAM-1的表达,构成了内毒损伤络脉的部分生物学基础。Objective To observe the influence of oxidized low-density lipoprotein （ox-LDL） on the activities of human brain microvascular endothelial cells （HBMEC） and expressions of intercellular adhesion molecule-1 （ICAM-1） and vascular cell adhesion molecule-1 （VCAM-1 investigate the modern molecular biology mechanism of brain collateral damage ） in vitro, and to by internal toxin. Methods HBMEC were cultured as the target cells and ox-LDL in different concentrations （0,5, 10, 25, 50, 75, 100 and 150 mg/L） were added into the endothelial cell medium for 24, 48 and 72 hours respectively. The cell activities were detected by using MTT assay, and different ox-LDL concentration groups （0, 50, 75 and 100 mg/L） were chosen at different time points （24 and 72 h） on base of MTT assay results. The changes of ICAM-1 and VCAM-1 were observed by using immunocytochemistry method and quantitative image analysis system. Results The influence of ox-LDL on the activities of HBMEC had time and concentration dependency with significant statistical difference. The results of immunocytochemistry method and quantitative image analysis system showed that the optical density values （OD） in different ox-LDL groups （50, 75 and 100 mg/L） were all different significantly （P 〈0.01 ） compared with those in the normal control group at two time points （24 and 72 hours）. There was no statistical difference in the same concentration group at two time points （24 and 72 hours ）. Conclusion ox-LDL in different concentrations can change the activities of HBMEC in vitro and promote the expressions of ICAM-1 and VCAM-1 with a concentration dependency, which forms a part of biological basis of brain collateral damage by internal toxin.

关 键 词：毒损脑络 人脑微血管内皮细胞 氧化低密度脂蛋白 细胞黏附分子 

分 类 号：R285.5[医药卫生—中药学]