薤白对气滞型血管损伤COX-2和iNOS含量及相互作用的影响  

Influence of Bulbus Allii Macrostemi on contents and interaction of COX-2 and iNOS in vascular endothelial injury of qi-stagnation type

作　　者：冀召帅[1] 吴以岭 贾振华 齐锦生[1] 

机构地区：[1]河北医科大学生物化学与分子生物学教研室,河北050017 [2]河北以岭医药研究院

出　　处：《北京中医药大学学报》2008年第12期835-838,867,868,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(No.2005CB523301)

摘　　要：目的探讨气滞型大鼠血管内皮损伤中环氧合酶-2(COX-2)和诱导型一氧化氮合酶(iNOS)的蛋白水平及相互作用,以及薤白的防治作用机制。方法用高L-蛋氨酸复制SD大鼠血管内皮损伤,并附以束缚法制造气滞实验模型。应用蛋白质印迹分析内皮损伤相关关键蛋白COX-2和iNOS蛋白水平的变化,免疫共沉淀和激光共聚焦显微镜技术,探讨COX-2和iNOS的相互作用,光镜和电镜分析血管内皮的病理变化。结果模型组血管COX-2和iNOS蛋白含量显著增高,并存在明显的相互作用,与血管内皮光镜和电镜下的病理损伤相一致;与模型组相比,薤白组COX-2和iNOS的蛋白含量降低,两者的相互作用减弱(P<0.05或P<0.01),且血管内皮的病理损伤明显减轻。结论气滞型大鼠血管内皮损伤中,COX-2和iNOS蛋白含量增高,且两者之间的相互作用增强,可加重血管内皮损伤,薤白能够纠正这些异常,起到保护血管内皮免受损伤的作用。Objective To investigate the protein contents and interaction of cyclooxygenase-2 （COX-2） and inducible nitric oxide synthase （iNOS） in rats with vascular endothelial injury of qi-stagnation type, and the mechanism of preventive and therapeutic effect of Bulbus Allii Macrostemi. Methods The model of qi-stagnation was established by using binding method in SD rats after coping vascular endothelial injury by high L-methionine. The changes of COX-2 and iNOS contents were analyzed by Western blotting method, the interaction of COX-2 and iNOS was investigated by co-immunoprecipitation and laser confocal microscopy, and the pathological changes of vascular endothelium were observed by the optical microscope and electron microscope. Results In the model group the contents of COX-2 and iNOS increased significantly and there was an interaction obviously, which was accorded with the pathological injury of vascular endothelium observed by the microscope and electron microscope. In the Bulbus Allii Macrostemi group the contents of COX-2 and iNOS decreased and their interaction was reduced compared with those in the model group （P 〈0.05 or P 〈0.01）, and the injury of vascular endothelium was alleviated significantly. Conclusion In vascular endothelial injury of qi stagnation type the protein contents of COX-2 and iNOS are increased and their interaction is enhanced, which aggravates the vascular endothelial injury. Bulbus Allii Macrostemi can remedy these disorders and protect the vascular endothelial cells from injury.

关 键 词：薤白 血管内皮损伤 环氧合酶-2 诱导型一氧化氮合酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]