高效液相色谱-示差折光检测法同时测定维生素C葡萄糖注射液中维生素C与葡萄糖的含量  

Simultaneous Assay of Vitamin C and Glucose in Vitamin C and Glucose Injection by HPLC with RID

作　　者：张云楚[1] 张新规[2] 

机构地区：[1]江苏省药品检验所,南京210008 [2]中国药科大学,南京210009

出　　处：《中国药品标准》2008年第6期421-424,共4页Drug Standards of China

摘　　要：目的:建立高效液相色谱-示差折光检测法测定维生素C葡萄糖注射液含量的方法。方法:采用示差折光检测器色谱柱采用氨基键合硅胶色谱柱(4.6mm×250mm,5μm);流动相为乙腈-0.02mol·L-1KH2PO4溶液(pH6.0)(75∶25);柱温30℃,流速为1.0mL·min-1。结果:维生素C和葡萄糖分别在0.10～1.00和0.25～2.50g.L-1的浓度范围内有良好的线性关系(r=0.9997);(r=0.9998),两者的平均回收率分别为100.2%与99.8%,RSD分别为1.5%与1.0%。结论:此法简便、快速、准确,适用于维生素C葡萄糖注射液的含量测定。Objective：To establish a method using HPLC - RID for simultaneous assay of Vitamin C and Glucose in Vitamin C and Glucose Injection. Methods：The detector was differential refraction detector. The Kromasil 100-5 NH2 （4. 6 mm × 250 mm,5 μm）set at 30℃ was used. The mobile phase was composed of acetonitrile-0. 02 mol · L^-1 KH2PO4 solution （ pH 6.0）. Results：The linear range of Vitamin C was 0. 10 - 1.00 g · L^-1 （r =0. 999 7） ,and Glucose was 0. 25 g · L^-2. 50 g · L^- （r =0. 999 7）. The average recovery of Vitamin C was 100. 2% （ RSD 1.5 % ）, and the average recovery of Glucose was 99. 8 % （ RSD 1.0 % ）. Conclusions ： This method is efficient, accurate and suitable for the simultaneous assay of Vitamin C and Glucose in Vitamin C and Glucose Injection.

关 键 词：维生素C 葡萄糖 含量测定 高效液相色谱 示差折光检测法 

分 类 号：R942[医药卫生—药剂学] R286[医药卫生—药学]