人参皂苷Rb_3对大鼠局灶性脑缺血损伤的保护作用  

Protective effects of ginsenoside Rb_3 on focal cerebral ischemic injury in rats

作　　者：胡瑜 陈浩凡 臧林泉[2] 巫玮[2] 

机构地区：[1]广东省边防总队医院药剂科,广东深圳518029 [2]广东药学院药科学院新药筛选中心,广东广州510006

出　　处：《广东药学院学报》2008年第6期590-593,共4页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的探讨3种人参皂苷Rb3对大鼠局灶性脑缺血损伤的保护作用。方法将48只大鼠随机分成6组:假手术组、模型组、人参皂苷Rb320、40、80 mg/kg组和阳性对照药血栓通注射液(XST)组;采用插线法使大脑中动脉闭塞(MCAO)建立大鼠局灶性脑缺血模型,于造模24 h后进行行为学评分,测定脑组织含水量以及脑组织中超氧化物歧化酶(SOD)和丙二醛(MDA)含量,计算脑梗死面积百分比,并进行组织病理学检查。结果人参皂苷Rb340、80 mg/kg能明显缓解脑缺血大鼠的行为学症状,减轻其脑水肿程度,降低血清中MDA含量,升高SOD含量,并能减少其脑梗死面积,减轻组织病理学损伤。结论人参皂苷Rb3对大鼠局灶性脑缺血损伤有较明显的保护作用。Objective To study the protective effects of ginsengoside Rb3 on focal cerebral ischemia in rats.Methods 48 SD rats were divided randomly into sham-operated group,model group,ginsenoside Rb3（20,40 and 80 mg/kg） group and positive control drug（XST） group.The model of focal cerebral ischemia was made by middle cerebral artery occlusion（MCAO） with nylon suture;neuroethological scores were gotten 24 h after the development of the model.The water content of brain tissue,the activities of SOD and MDA in brain tissue and were measured,the percentage of cerebral infarction area to total brain area was calculated,and histopathological examination was conducted.Results Ginsenoside Rb3（at 40 mg/kg and 80 mg/kg） could significantly reduce the scores for neurological deficits and brain edema,improve the activities of SOD,lower the content of MDA,reduce the cerebral infraction area,and alleviate the cerebral ischemia injury.Conclusion Ginsenoside Rb3 had obvious protective effects on the focal cerebral ischemia in rats.

关 键 词：脑缺血 大脑中动脉闭塞(MCAO) 人参皂苷RB3 

分 类 号：R285.5[医药卫生—中药学]