AFB1 Bio-Degradation by a New Strain - Stenotrophomonas. sp  

作　　者：LIANG Zhi-hong LI Jun-xia HE Yun-long GUAN Shu WANG Nin JI Cheng NIU Tian-gui 

机构地区：[1]College of Food Science ＆ Nutritional Engineering, China Agricultural University, Beijing 100083, P.R.China [2]College of Animal Science and Technology, China Agricultural University, Beijing 100193, P.R.China

出　　处：《Agricultural Sciences in China》2008年第12期1433-1437,共5页中国农业科学（英文版）

基　　金：the Ministry of Science and Technology of China (2005DKA21204-11);the Na-tional Natural Science Foundation of China (30571353);the National High Technology Research and Devel-opment Program of China (863 Program,2006AA10Z442)

摘　　要：The paper was to find the bacteria to degrade aflatoxin B 1 （AFB 1） and realize the application of biological degradation on AFB 1. Using cumarin as the carbon source and energy on the first screening, then the ten strains which were first screened out were taken to degrade AFB 1 100 pg kg^-1. Strain NMO-3 was screened out of ten strains, the degradation ratio of AFB 1 reached 85.7%, which was more prominent than the others （P 〈 0.01）. With the analysis of colony morphology, physiological and biochemistry experiments, and 16S rDNA gene sequence, the strain NMO-3 was finally identified as Stenotrophomonas sp. Using cumarin as the carbon source and energy could screen out the AFB 1 degradation strains. Acute toxicity tests show that the viable number of NMO-3 lower than 3.12 × 10^10 cfu mL-1 is safety. The crude enzyme was obtained by 65% ammonium sulfate fractionation, and it could degrade AFB1. It is the first report for the strain＇s detoxi- AFB1.The paper was to find the bacteria to degrade aflatoxin B 1 （AFB 1） and realize the application of biological degradation on AFB 1. Using cumarin as the carbon source and energy on the first screening, then the ten strains which were first screened out were taken to degrade AFB 1 100 pg kg^-1. Strain NMO-3 was screened out of ten strains, the degradation ratio of AFB 1 reached 85.7%, which was more prominent than the others （P 〈 0.01）. With the analysis of colony morphology, physiological and biochemistry experiments, and 16S rDNA gene sequence, the strain NMO-3 was finally identified as Stenotrophomonas sp. Using cumarin as the carbon source and energy could screen out the AFB 1 degradation strains. Acute toxicity tests show that the viable number of NMO-3 lower than 3.12 × 10^10 cfu mL-1 is safety. The crude enzyme was obtained by 65% ammonium sulfate fractionation, and it could degrade AFB1. It is the first report for the strain＇s detoxi- AFB1.

关 键 词：AFLATOXIN biological degradation identification Stenotrophomonas sp. 

分 类 号：S13[农业科学—农业基础科学]