银杏叶提取物对溶血磷脂酰胆碱引起的脑微血管细胞损伤的保护作用  

Protective effect of Ginkgo biloba extract on the injury in cerebral microvascular cell by lysophosphatidylcholine

作　　者：李菲[1] 芮耀诚[1] 陈维洲 

机构地区：[1]中国人民解放军第二军医大学药学院药理教研室 [2]中国科学院上海药物研究所

出　　处：《中国新药与临床杂志》1998年第3期149-151,共3页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的：研究银杏叶提取物（ＧｂＥ）对溶血磷脂酰胆碱（ＬＰＣ）引起的小牛脑微血管内皮细胞（ＢＣＭＥＣ）损伤及小牛脑微血管平滑肌细胞（ＢＣＭＳＭＣ）增殖的保护作用。方法：以乳酸脱氢酶（ＬＤＨ）释放量为指标测定ＢＣＭＥＣ损伤，用结晶紫染色法测定ＢＣＭＳＭＣ增殖。结果：ＬＰＣ５０ｎｍｏｌ／Ｌ可明显增加ＬＤＨ的释放，ＧｂＥ０，５，１０，２０，５０和１００ｍｇ／Ｌ，则ＬＤＨ释放率由４７．０％±０．７％分别下降为３９％±３％，３６．０％±１．３％，２７．０％±２．１％，１６％±６％和１３％±３％，表明ＧｂＥ呈剂量依赖性地抑制了ＬＤＨ的释放。ＧｂＥ对ＬＰＣ引起的ＢＣＭＳＭＣ的增殖抑制作用不明显。AIM: To study the effects of protetion of Ginkgo biloba extract (GbE) on lactic dehydrogenase (LDH) release from bovine cerebral microvascular endothelial cells (BCMEC) and proliferation in bovine cerebral microvascular smooth muscle cells (BCMSMC) by lysophosphatidylcholine (LPC). METHODS: The injury of BCMEC was determined by the LDH release. The proliferation of BCMSMC was determined with crystal violet. RESULTS: GbE (5, 10, 20, 50 and 100 mg/L) inhibited LDH release by LPC (50 nmol/L) in a concentration_depen_ dent manner. The LDH release was reduced from 47.0%±0.7% (control) to 39%±3%, 36.0± 1.3%, 27.0±2.1%, 16±6%, 13±3%, respectively. GbE did not significantly antogonize the proliferation effect by LPC in cultured BCMSMC. CONCLUSION: GbE protects BCMEC injury by LPC.

关 键 词：银杏提取物 脑血管损伤 微血管 溶血磷脂酰胆碱 

分 类 号：R277.73[医药卫生—中医学]