微生物转化植物甾醇为雄烯二酮检测方法的建立  

Method for Detecting the Transformation of Phytosterol to Androst-4-ene-3,17-dione with Microorganism

作　　者：杨英[1,2] 姜绍通[2] 赵俊平[2] 胡锦艳[2] 操丽丽[2] 

机构地区：[1]安徽建筑工业学院环境工程系,安徽合肥230022 [2]合肥工业大学生物与食品学院

出　　处：《食品与发酵工业》2008年第11期152-155,共4页Food and Fermentation Industries

基　　金：国家"十一五"攻关项目(2006BAD05A12);安徽省自然科学基金项目(070413265X)

摘　　要：利用植物甾醇和雄烯二酮的标品以及发酵液作为待测样品,比较了薄层色谱法,紫外可见光分光光度法,高效液相色谱法和气相色谱法4种分析方法,得出分析效果最好的方法是气相色谱法;结合生产的实际情况,建立了微生物转化植物甾醇为雄烯二酮的检测方法,先用TLC薄层层析法分析,后有选择性的进行气相色谱分析。Four analytical methods including TLC, UV, HPLC and GC were compared on the detecting of the transformation of phytosterol to androst-4-ene-3,17-dione in fermentation samples. The standard samples were phytosterol and androst-4-ene-3. The results showed that the best method was GC. For the practical application, samples should be treated with TLC first and then selectively analyzed by GC.

关 键 词：雄烯二酮 薄层色谱法 紫外可见光分光光度法 高效液相色谱法 气相色谱法 

分 类 号：TQ460.72[医药卫生—药物分析学]