毛细管电泳-电化学检测测定野菊花中的黄酮类化合物  

Determination of flavonoids in Flos chrysanthemi indici by capillary electrophoresis with electrochemical detection

作　　者：许雪琴[1] 余丽双[1] 陈国南[1] 

机构地区：[1]福州大学化学化工学院,食品安全分析与检测技术教育部重点实验室,福建福州350002

出　　处：《福州大学学报（自然科学版）》2008年第6期884-887,共4页Journal of Fuzhou University(Natural Science Edition)

基　　金：福建省自然科学基金资助项目(2007J0130);福建省高等学校新世纪优秀人才支持计划资助项目(XSJRC2007-13)

摘　　要：采用毛细管电泳-电化学检测测定了野菊花中3种黄酮类化合物（刺槐素、槲皮素和木犀草素）的含量.研究了检测电位、运行缓冲溶液浓度和pH值,分离电压和进样时间对分离和检测的影响.以微碳圆盘电极（Ф=0.5 mm）为工作电极,检测电位为＋0.95 V（vs.Ag/AgCl）,以pH=8.00的50 mmol/L Na2B4O7~100mmol/L NaH2PO4缓冲液为运行液,当分离电压为21 kV时,3种黄酮类化合物在16 min内完全分离.刺槐素、槲皮素和木犀草素的线性范围分别为0.73~22.2、0.23~16.0和0.29~18.2μg/mL;检出限（S/N=3）分别为0.36、0.16和0.08μg/mL.该方法已成功地应用于野菊花中3种黄酮类化合物的测定.Capillary electrophoresis with electrochemical detection was used to determine three flavonoids （acacetin, quercetin and luteolin ）. The influences of several important factors such as pH and concentration of running buffer, separation voltage, injection time and detection potential were investigated to acquire the optimum conditions. With a 50 mmol/L Na2B407 - 100 mmol/L NaH2PO4 buffer （ pH = 8.00） as running buffer, three flavonoids were separated completely within 16min in a 60 cm length capillary at a separation voltage of 21 kV. The linear ranges for acacetin, quercetin and luteolin are 0. 73 -22.2, 0.23 - 16.0 and 0. 29 - 18.2 μg/mL, respectively, and the limit detections are 0.36, 0.16 and 0.08 μg/mL, respectively. This method was used to determine the above three flavonoids in enthanolic extract of Flos chrysanthemi indici, and the assay results were satisfactory.

关 键 词：毛细管电泳 电化学检测 野菊花 黄酮类化合物 

分 类 号：O657.8[理学—分析化学]